303 388

Cited 75 times in

A role for O-GlcNAcylation in setting circadian clock speed

DC Field Value Language
dc.contributor.authorKim, EY-
dc.contributor.authorJeong, EH-
dc.contributor.authorPark, S-
dc.contributor.authorJeong, HJ-
dc.contributor.authorEdery, I-
dc.contributor.authorCho, JW-
dc.date.accessioned2013-04-24T06:31:15Z-
dc.date.available2013-04-24T06:31:15Z-
dc.date.issued2012-
dc.identifier.issn0890-9369-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/7943-
dc.description.abstractPost-translational modifications of one or more central "clock" proteins, most notably time-of-day-dependent changes in phosphorylation, are critical for setting the pace of circadian (≅24 h) clocks. In animals, PERIOD (PER) proteins are the key state variable regulating circadian clock speed and undergo daily changes in abundance and cytoplasmic-nuclear distribution that are partly driven by a complex phosphorylation program. Here, we identify O-GlcNAcylation (O-GlcNAc) as a critical post-translational modification in circadian regulation that also contributes to setting clock speed. Knockdown or overexpression of Drosophila O-GlcNAc transferase (ogt) in clock cells either shortens or lengthens circadian behavioral rhythms, respectively. The Drosophila PERIOD protein (dPER) is a direct target of OGT and undergoes daily changes in O-GlcNAcylation, a modification that is mainly observed during the first half of the night, when dPER is predominantly located in the cytoplasm. Intriguingly, the timing of when dPER translocates from the cytoplasm to the nucleus is advanced or delayed in flies, wherein ogt expression is reduced or increased, respectively. Our results suggest that O-GlcNAcylation of dPER contributes to setting the correct pace of the clock by delaying the timing of dPER nuclear entry. In addition, OGT stabilizes dPER, suggesting that O-GlcNAcylation has multiple roles in circadian timing systems.-
dc.language.isoen-
dc.subject.MESHAcylation-
dc.subject.MESHAnimals-
dc.subject.MESHCasein Kinase Iepsilon/metabolism-
dc.subject.MESHCell Line-
dc.subject.MESHCell Nucleus/metabolism-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCircadian Clocks/*physiology-
dc.subject.MESHDrosophila Proteins/metabolism-
dc.subject.MESHDrosophila melanogaster/metabolism/*physiology-
dc.subject.MESHGene Expression Regulation, Enzymologic-
dc.subject.MESHGene Knockdown Techniques-
dc.subject.MESHN-Acetylglucosaminyltransferases/metabolism-
dc.subject.MESHNeurons/enzymology/metabolism-
dc.subject.MESHPeriod Circadian Proteins/metabolism-
dc.subject.MESHRNA, Messenger/metabolism-
dc.titleA role for O-GlcNAcylation in setting circadian clock speed-
dc.typeArticle-
dc.identifier.pmid22327476-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3305986/-
dc.contributor.affiliatedAuthor김, 은영-
dc.type.localJournal Papers-
dc.identifier.doi10.1101/gad.182378.111-
dc.citation.titleGenes & development-
dc.citation.volume26-
dc.citation.number5-
dc.citation.date2012-
dc.citation.startPage490-
dc.citation.endPage502-
dc.identifier.bibliographicCitationGenes & development, 26(5):490-502, 2012-
dc.identifier.eissn1549-5477-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Brain Science
Files in This Item:
22327476.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse