57 243

Cited 41 times in

Analysis of changes in gene expression and metabolic profiles induced by silica-coated magnetic nanoparticles

Authors
Shim, W; Paik, MJ; Nguyen, DT; Lee, JK; Lee, Y; Kim, JH; Shin, EH; Kang, JS; Jung, HS; Choi, S; Park, S; Shim, JS; Lee, G
Citation
ACS nano, 6(9):7665-7680, 2012
Journal Title
ACS nano
ISSN
1936-08511936-086X
Abstract
Magnetic nanoparticles (MNPs) have proven themselves to be useful in biomedical research; however, previous reports were insufficient to address the potential dangers of nanoparticles. Here, we investigated gene expression and metabolic changes based on the microarray and gas chromatography-mass spectrometry with human embryo kidney 293 cells treated with MNPs@SiO(2)(RITC), a silica-coated MNP containing Rhodamine B isothiocyanate (RITC). In addition, measurement of reactive oxygen species (ROS) and ATP analysis were performed to evaluate the effect of MNPs@SiO(2)(RITC) on mitochondrial function. Compared to the nontreated control, glutamic acid was increased by more than 2.0-fold, and expression of genes related to the glutamic acid metabolic pathway was also disturbed in 1.0 μg/μL of MNPs@SiO(2)(RITC)-treated cells. Furthermore, increases in ROS concentration and mitochondrial damage were observed in this MNPs@SiO(2)(RITC) concentration. The organic acids related to the Krebs cycle were also disturbed, and the capacity of ATP synthesis was decreased in cell treated with an overdose of MNPs@SiO(2)(RITC). Collectively, these results suggest that overdose (1.0 μg/μL) of MNPs caused transcriptomic and metabolic disturbance. In addition, we suggest that a combination of gene expression and metabolic profiles will provide more detailed and sensitive toxicological evaluation for nanoparticles.
MeSH terms
Coated Materials, Biocompatible/*administration & dosageDose-Response Relationship, DrugGene Expression Regulation/*drug effects/*physiologyHEK293 CellsHumansMagnetite Nanoparticles/*administration & dosageMaterials TestingMetabolome/*physiologyProteome/*metabolismSilicon Dioxide/*administration & dosage
DOI
10.1021/nn301113f
PMID
22830605
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
이, 광
Files in This Item:
fulltext not available.txtDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse