Cited 0 times in Scipus Cited Count

Chiral separation of N-methyl-DL-aspartic acid in rat brain tissue as N-ethoxycarbonylated (S)-(+)-2-octyl ester derivatives by GC-MS

DC Field Value Language
dc.contributor.authorNguyen, DT-
dc.contributor.authorKim, KR-
dc.contributor.authorLee, G-
dc.contributor.authorPaik, MJ-
dc.date.accessioned2013-04-25T03:29:13Z-
dc.date.available2013-04-25T03:29:13Z-
dc.date.issued2012-
dc.identifier.issn0269-3879-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/7977-
dc.description.abstractA selective and sensitive analytical method was developed for enantiomeric separation and determination of N-methyl-DL-aspartic acid (NMA). The method involved the conversion of each enantiomer into N-ethoxycarbonylated (S)-(+)-2-octyl ester derivative for the direct separation by gas chromatography-mass spectrometry (GC-MS). The diastereomeric derivatives showed characteristic mass spectral properties for analysis by selected ion monitoring mode (SIM) and enabling enantioseparation on an achiral capillary column. Two enantiomers were baseline separated, and the detection limits for N-methyl-L-aspartic acid (NMLA) and N-methyl-D-aspartic acid (NMDA) were 0.07 and 0.03 ng/g, respectively. When applied to rat brain tissues for absolute configuration of NMA, only NMDA was determined, while NMLA was monitored as lower than the limit of detection.-
dc.formatapplication/pdf-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHBrain Chemistry-
dc.subject.MESHEsters-
dc.subject.MESHGas Chromatography-Mass Spectrometry-
dc.subject.MESHLimit of Detection-
dc.subject.MESHN-Methylaspartate-
dc.subject.MESHRats-
dc.subject.MESHReproducibility of Results-
dc.subject.MESHStereoisomerism-
dc.titleChiral separation of N-methyl-DL-aspartic acid in rat brain tissue as N-ethoxycarbonylated (S)-(+)-2-octyl ester derivatives by GC-MS-
dc.typeArticle-
dc.identifier.pmid22290726-
dc.contributor.affiliatedAuthor이, 광-
dc.type.localJournal Papers-
dc.identifier.doi10.1002/bmc.2703-
dc.citation.titleBiomedical chromatography : BMC-
dc.citation.volume26-
dc.citation.number11-
dc.citation.date2012-
dc.citation.startPage1353-
dc.citation.endPage1356-
dc.identifier.bibliographicCitationBiomedical chromatography : BMC, 26(11). : 1353-1356, 2012-
dc.identifier.eissn1099-0801-
dc.relation.journalidJ002693879-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
Files in This Item:
There are no files associated with this item.

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse