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Protective effect of grape seed extract against oxidative stress-induced cell death in a staurosporine-differentiated retinal ganglion cell line

DC Field Value Language
dc.contributor.authorYang, H-
dc.contributor.authorLee, BK-
dc.contributor.authorKook, KH-
dc.contributor.authorJung, YS-
dc.contributor.authorAhn, J-
dc.date.accessioned2013-04-29T02:26:37Z-
dc.date.available2013-04-29T02:26:37Z-
dc.date.issued2012-
dc.identifier.issn0271-3683-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/8059-
dc.description.abstractOBJECTIVE: Grape seed extract (GSE) is a potent antioxidant. We examined the effect of GSE on oxidative stress-induced cell death in a transformed retinal ganglion cell line, RGC-5.



METHODS: Staurosporine-differentiated RGC-5 (ssdRGC-5) cells obtained by treating RGC-5 cells with 1 µM staurosporine were incubated with GSE for 2 h and then exposed to buthionine sulfoximine plus glutamate (B/G) for 24 h. Cell death was detected using the LIVE/DEAD viability assay and the type of cell death was evaluated using fluorescein isothiocyanate-conjugated Annexin-V/propidium iodide staining. To investigate the mechanism underlying cell death, we determined the caspase-3 activity and level of reactive oxygen species (ROS) formation.



RESULTS: Treatment of ssdRGC-5 cells with B/G increased intracellular ROS and induced apoptosis (not necrosis) with increasing caspase-3 activity. GSE rescued the ssdRGC-5 cells from oxidative stress-induced cell death by inhibiting both intracellular ROS production and caspase-3 activation.



CONCLUSION: GSE had a neuroprotective effect against oxidative stress-induced apoptotic death in ssdRGC-5 cells.
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dc.language.isoen-
dc.subject.MESHAntioxidants/*pharmacology-
dc.subject.MESHCaspase 3/drug effects/metabolism-
dc.subject.MESHCell Death/*drug effects-
dc.subject.MESHCell Line-
dc.subject.MESHCulture Media-
dc.subject.MESHEnzyme Inhibitors/pharmacology-
dc.subject.MESHFluorometry-
dc.subject.MESHGrape Seed Extract/*pharmacology-
dc.subject.MESHHumans-
dc.subject.MESHIntracellular Fluid/metabolism-
dc.subject.MESHOxidative Stress/*drug effects-
dc.subject.MESHReactive Oxygen Species/metabolism-
dc.subject.MESHRetinal Ganglion Cells/drug effects/*pathology-
dc.subject.MESHStaurosporine/pharmacology-
dc.titleProtective effect of grape seed extract against oxidative stress-induced cell death in a staurosporine-differentiated retinal ganglion cell line-
dc.typeArticle-
dc.identifier.pmid22440165-
dc.identifier.urlhttp://informahealthcare.com/doi/abs/10.3109/02713683.2011.645106-
dc.contributor.affiliatedAuthor양, 홍석-
dc.contributor.affiliatedAuthor국, 경훈-
dc.contributor.affiliatedAuthor안, 재홍-
dc.type.localJournal Papers-
dc.identifier.doi10.3109/02713683.2011.645106-
dc.citation.titleCurrent eye research-
dc.citation.volume37-
dc.citation.number4-
dc.citation.date2012-
dc.citation.startPage339-
dc.citation.endPage344-
dc.identifier.bibliographicCitationCurrent eye research, 37(4):339-344, 2012-
dc.identifier.eissn1460-2202-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Ophthalmology
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