53 196

Cited 0 times in

Quantitative lateral-flow immunoassay for the assessment of the cartilage oligomeric matrix protein as a marker of osteoarthritis

Hong, SY; Park, YM; Jang, YH; Min, BH; Yoon, HC
Biochip journal, 6(3):213-220, 2012
Journal Title
Biochip journal
This study reports a simple and quantitative analytical method for detecting cartilage oligomeric matrix protein (COMP) using a lateral-flow immunoassay (LFI) format. An immuno-chromatographic assay was developed using a gold nanoparticle (AuNP)-conjugated monoclonal antibody (Ab) probe for the quantitative detection of COMP in human synovial fluid (SF). The detection antibody was conjugated with the AuNP (∼30 nm) to enable detection, and the capture antibody was immobilized within a multi-spot pattern in the test zone of a membrane strip. COMP in the human SF binds to the AuNP-conjugated detection antibody, and the COMP-Ab complex then binds to the capture antibody. The immunoassay test can be analyzed by visualizing the test strip with a commercial scanner and an imaging software program. The color density of the multi-spot pattern at the test zone increased in proportion to the concentration of COMP. The developed immunoassay exhibited a dynamic detection range from 0.6 to 20 μg/mL of COMP. The detection limit of the proposed LFI system was approximately 0.2 μg/mL. The test results showed good correlation with those obtained using a conventional enzyme-linked immunosorbent assay (ELISA) to detect COMP. We expect that the proposed immunoassay method, based on the quantification of COMP, is suitable for osteoarthritis diagnosis using SF samples.
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
AJOU Authors
민, 병현
Files in This Item:
fulltext not available.txtDownload
RIS (EndNote)
XLS (Excel)


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.