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Chip-based comparison of the osteogenesis of human bone marrow- and adipose tissue-derived mesenchymal stem cells under mechanical stimulation

Authors
Park, SH; Sim, WY; Min, BH; Yang, SS; Khademhosseini, A; Kaplan, DL
Citation
PloS one, 7(9):e46689-e46689, 2012
Journal Title
PloS one
ISSN
1932-6203
Abstract
Adipose tissue-derived stem cells (ASCs) are considered as an attractive stem cell source for tissue engineering and regenerative medicine. We compared human bone marrow-derived mesenchymal stem cells (hMSCs) and hASCs under dynamic hydraulic compression to evaluate and compare osteogenic abilities. A novel micro cell chip integrated with microvalves and microscale cell culture chambers separated from an air-pressure chamber was developed using microfabrication technology. The microscale chip enables the culture of two types of stem cells concurrently, where each is loaded into cell culture chambers and dynamic compressive stimulation is applied to the cells uniformly. Dynamic hydraulic compression (1 Hz, 1 psi) increased the production of osteogenic matrix components (bone sialoprotein, oateopontin, type I collagen) and integrin (CD11b and CD31) expression from both stem cell sources. Alkaline phosphatase and Alrizarin red staining were evident in the stimulated hMSCs, while the stimulated hASCs did not show significant increases in staining under the same stimulation conditions. Upon application of mechanical stimulus to the two types of stem cells, integrin (β1) and osteogenic gene markers were upregulated from both cell types. In conclusion, stimulated hMSCs and hASCs showed increased osteogenic gene expression compared to non-stimulated groups. The hMSCs were more sensitive to mechanical stimulation and more effective towards osteogenic differentiation than the hASCs under these modes of mechanical stimulation.
MeSH terms
Abdominal Fat/*cytologyAdultAlkaline Phosphatase/metabolismAntigens, CD29/genetics/metabolismAntigens, Differentiation/genetics/metabolismBiomechanicsBone Marrow Cells/metabolism/*physiologyCell Culture Techniques*Cell DifferentiationCells, CulturedCollagen Type I/metabolismCore Binding Factor Alpha 1 Subunit/genetics/metabolismFemaleHumansHydrostatic PressureIntegrin-Binding Sialoprotein/genetics/metabolismMaleMesenchymal Stromal Cells/metabolism/*physiologyMicrofluidic Analytical Techniques/*instrumentationOsteogenesisOsteopontin/genetics/metabolismStress, Physiological
DOI
10.1371/journal.pone.0046689
PMID
23029565
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
AJOU Authors
민, 병현
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