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Role of JAK3 in myogenic differentiation

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dc.contributor.authorJang, YN-
dc.contributor.authorLee, IJ-
dc.contributor.authorPark, MC-
dc.contributor.authorBaik, EJ-
dc.date.accessioned2013-05-03T05:48:04Z-
dc.date.available2013-05-03T05:48:04Z-
dc.date.issued2012-
dc.identifier.issn0898-6568-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/8245-
dc.description.abstractSkeletal muscle differentiation is regulated by transcription factors, including members of the myogenic regulatory factor (MRF) family and many signaling pathways. The JAK1 and JAK2 pathways are known to each have different effects on myoblast proliferation and differentiation; however, the role of JAK3 in myoblast differentiation remains unclear. In this study, we investigated the effect of JAK3 inhibition on myogenic differentiation in the C2C12 mouse myoblast cell line. During myogenic differentiation, treatment with the JAK3 inhibitor WHIp154 significantly increased the number of MHC-positive multinucleated myotubes and the expressions of myosin heavy chain (MHC), myogenin (MGN), MyoD, and myogenic enhancer factor 2 (MEF2). Knockdown of the JAK3 gene using siJAK3 also significantly increased MHC, MGN and MyoD mRNA expressions as well as insulin-like growth factor-II (IGF-II) gene expression. During differentiation, JAK3 was initially activated and later decreased. Differentiation decreased STAT1, which was further decreased by WHIp154. In contrast, STAT3 gradually was elevated during differentiation, and was increased by JAK3 inhibition. Moreover, we found that up-regulation of AKT activity and down-regulation of ERK activity cooperated to accelerate myogenic differentiation. Taken together, these data indicate that JAK3 inhibition potently facilitates myoblast differentiation through antagonistic STAT1/STAT3 activities. Additionally, JAK3 inhibition induced precocious differentiation and played important roles for terminal differentiation, including fusion, which is involved with regulation of AKT and ERK pathways.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHCell Differentiation-
dc.subject.MESHCell Line-
dc.subject.MESHInsulin-Like Growth Factor II-
dc.subject.MESHJanus Kinase 3-
dc.subject.MESHMAP Kinase Signaling System-
dc.subject.MESHMice-
dc.subject.MESHMyoD Protein-
dc.subject.MESHMyoblasts-
dc.subject.MESHMyogenic Regulatory Factors-
dc.subject.MESHMyogenin-
dc.subject.MESHMyosin Heavy Chains-
dc.subject.MESHQuinazolines-
dc.subject.MESHRNA Interference-
dc.subject.MESHRNA, Small Interfering-
dc.subject.MESHSTAT1 Transcription Factor-
dc.subject.MESHSTAT3 Transcription Factor-
dc.titleRole of JAK3 in myogenic differentiation-
dc.typeArticle-
dc.identifier.pmid22120524-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0898-6568(11)00355-X-
dc.contributor.affiliatedAuthor장, 유나-
dc.contributor.affiliatedAuthor이, 일재-
dc.contributor.affiliatedAuthor박, 명철-
dc.contributor.affiliatedAuthor백, 은주-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.cellsig.2011.11.009-
dc.citation.titleCellular signalling-
dc.citation.volume24-
dc.citation.number3-
dc.citation.date2012-
dc.citation.startPage742-
dc.citation.endPage749-
dc.identifier.bibliographicCitationCellular signalling, 24(3). : 742-749, 2012-
dc.identifier.eissn1873-3913-
dc.relation.journalidJ008986568-
Appears in Collections:
Journal Papers > Research Organization > Inflamm-aging Translational Research Center
Journal Papers > School of Medicine / Graduate School of Medicine > Plastic & Reconstructive Surgery
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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