Reinforcement fibrin-hyaluronic acid composite gel for tissue engineering cartilage genesis

Abstract

The reimplantation method of cultured chondrocytes broadly has been offered as an alternative for articular cartilage repair. A variety of biologically derived and synthetic polymeric and hydrogel materials also have been investigated for good cell delivery efficiency. Preciously, we examined the feasibility of fibrin gel, mixed with hyaluronic acid(HA) as a cell delivery carrier. In order to reinforce the material, hybrid biomaterials of fibrin/HA composite gels with fibrinolysis inhibition factors(FIFs: aprotinin, DI101, EACA) have been investigated in the present work because we did not satisfy a little progress. These fibrin/HA composite gels added FIFs maintained their structural integrity in long-term culture over 4th weeks. Contrary to our expectation the mass of the fibrin/HA composite with DI 101 was significantly superior to the ones of other combinations. In histological evidence, all of them are showed good positive result of stain of Safranin-O and alcian blue during the culture period. In gross examination, samples of all groups grossly resembled cartilage in color and were resistant to external compression. Our study demonstrates that most favorable polymer can be used good quality tissue engineered cartilage and in this culture systems have been useful for studying the basic biology of chondrocyte biosynthesis of ECM and new cartilage matrix formation without a loss of volume. After all, we proved the safety of inhibitors of the fibrinolytic system without hazardous effect on cell behavior and found out that DI 101 would be the most effective agent.