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Chitosan/heparin polyelectrolyte complex nanoparticles (100-200nm) covalently bonded with PEI for enhancement of chondrogenic phenotype

Authors
Lee, HJ; Park, KH; Park, SR; Min, BH
Citation
Key engineering materials, 342-343:329-332, 2007
Journal Title
Key engineering materials
ISSN
1013-9826
Abstract
Acknowledging the supportive influence of matrix molecules on the chondrocytic phenotype, we combined heparin and chitosan to develop a novel biomaterial, supporting chondrogenesis. Chitosan had been shown as a promising structural material for a number of tissue engineering applications. Similar to heparin, one of the glycosamino-glycans (GAGs) had been known to exert various influences on the biological activities. In this study we evaluated the potential of heparin/chitosan polyelectrolyte complex materials for controlling the proliferation and differentiation of chondrocytes. The heparin/chitosan polyelectrolyte complex was coated over the polyethyleneimine (PEI) layer precoated on the commercial polystyrene dish. When examined by using human chondrocyte cell line (C28/I2), the heparin/chitosan surfaces supported well not only the cell proliferation but also the chondrocytic functions, such as expression of collagen type II. These results indicated that the heparin/chitosan complex could be used to modulate the activity of chondrocytes in vitro.
Keywords
ChitosanHeparinNanoparticle culture systemPolyethylenimine (PEI)
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
AJOU Authors
민, 병현
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