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Inclusion Body Formation and Apoptotic Cell Death in the Human Neural Stem Cells HB1.F3 Following Gene Transfection of Alpha-Synuclein and Synphilin-1

Other Title
인간 신경 줄기세포 HB1.F3에서 alpha-synuclein과 synphilin-1 유전자 전이를 통한 봉입체 형성과 세포 자멸사
Authors
천, 상명; 이, 광; 김, 재우; 김, 승업
Citation
Taehan Sin'gyŏngkwa Hakhoe chi : sin'gyŏnghak, 25(3):344-352, 2007
Journal Title
Taehan Sin'gyŏngkwa Hakhoe chi : sin'gyŏnghak; Journal of the Korean Neurological Association : neurology
ISSN
1225-70442288-985X
Abstract
Background:The etiology of Parkinson’s disease (PD) has not been established, but familial forms of the disease

have some clues for its pathogenesis. Autosomal dominantly inherited familial PD induced by aberrations of the

alpha-synucein gene has been known as a genetic model of PD and sheds light to the understanding of PD

pathogenesis. Synphilin-1 is a protein which interacts with alpha-synuclein and constitutes the Lewy body.



Methods:Immortalized human neural stem cells were transfected with the alpha-synuclein gene and synphilin-1

gene, to define the role of Lewy body inclusions in neuronal cell death.



Results:Human neural stem cells with Lewy body-like inclusions showed an increased apoptotic cell death

compared to those with diffuse alpha-synuclein-positive and synphilin-1-positive reaction after transfection with

the alpha-synuclein gene and synphilin-1 gene. Tyrosine hydroxylase over-expressing cells produced a high level

of levodopa and showed a higher rate of the apoptotic marker.



Conclusions:These results suggest that the formation of Lewy body-like inclusions by the over-expression of

alpha-synuclein and synphilin-1 could be an underlying cause of apoptotic neuronal cell death and the dopaminergic cell might be more susceptible.
Keywords
Parkinson’s diseaseAlpha-synucleinLewybodyLevodopaApoptosis
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Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
이, 광
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