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The effects of propofol on neurotoxicity induced by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid in rat mixed cortical cultures

Other Title
백서 피질 신경원 혼합배양 모델에서 α-amino-5-methyl-4-isoxazolepropionate로 유도된 뇌독성에 대한 Propofol의 효과
Authors
서, 명신; 박, 성용; 김, 계숙; 문, 봉기; 김, 진수; 이, 숙영
Citation
Taehan Mach'ikwa Hakhoe chi, 55(5):607-612, 2008
Journal Title
Taehan Mach'ikwa Hakhoe chi; The Journal of the Korean Society of Anesthesiologists; 대한마취과학회지
ISSN
0302-5780
Abstract
Background

The pattern of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-mediated neurotoxicity (necrosis vs apoptosis) and the neuroprotective effect of propofol on AMPA-mediated neurotoxicity are still unclear.



Methods

Thirteen-day-old primary rat mixed cortical cultures were used. To measure the neuroprotective effect of propofol, AMPA (50µM), AMPA (50µM) plus propofol (0.1, 1, 25, 50µM), AMPA (50µM) plus DMSO, propofol (50µM) and DMSO were administered (n = 45). Seventy-two h later, surviving cells were counted using trypan blue staining and were converted to cell death rate (CDR). To measure the effect of propofol (50µM) on AMPA (50µM)-induced apoptosis, a triple stain was done. In a fixed field (×400), the number of neuronal cells stained by neuronal nuclei (NeuN) and Hoechst staining and apoptotic cells stained by terminal deoxynucleotidyl transferase mediated dUTP nick-end-labeling (TUNEL) assays were counted. Apoptotic cell rates (ACR) were also calculated. Statistical analyses were performed using one way-analysis of variance followed by Bonferroni's test. P < 0.05 was considered statistically significant.



Results

AMPA (50µM) stimulation demonstrated 49.3% CDR, and adding propofol 50µM decreased CDR to 29.4% (P < 0.05). In the TUNEL assay, cells with no drug treatment demonstrated 12.3% ACR and 50µM AMPA increased ACR to 28% (P < 0.05). Adding 50µM propofol to AMPA decreased the ACR to 20.1% (P < 0.05).



Conclusions

Propofol (50µM) had neuroprotective effects against AMPA (50µM)-induced cell death by reducing apoptosis.
Keywords
α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acidapoptosispropofol
DOI
10.4097/kjae.2008.55.5.607
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anesthesiology & Pain Medicine
AJOU Authors
박, 성용문, 봉기김, 진수이, 숙영
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