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Generation and Characterization of Monoclonal Antibodies against Human Interferon-lambda1

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dc.contributor.authorHong, SH-
dc.contributor.authorKim, JS-
dc.contributor.authorPark, S-
dc.date.accessioned2014-01-20T01:50:24Z-
dc.date.available2014-01-20T01:50:24Z-
dc.date.issued2008-
dc.identifier.issn1598-2629-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/8992-
dc.description.abstractBackground:Members belonging to the interferon-lambda (IFN-?) family exert protective action against viral infection; however, the mechanisms of their action have remained elusive. To study IFN-? biology, such as endocytosis of IFN-?, we produced monoclonal antibodies (Abs) against human IFN-? and examined their usefulness.



Methods:We purified recombinant human IFN-?1 expressed in Escherichia coli by using affinity columns. Then, we generated hybridoma cells by fusing myeloma cells with splenocytes from IFN-?1- immunized mice. For evaluating the neutralizing activity of the monoclonal Abs against IFN-?1, we performed RT-PCR for the MxA transcript. In order to study the binding activity of IFN-? and the monoclonal Ab complex on HepG2 cells, we labeled the monoclonal Ab with rhodamine and determined the fluorescence intensity.



Results:Four hybridoma clones secreting Abs specific to IFN-?1 were generated and designated as HL1, HL2, HL3, and HL4. All the Abs reacted with IFN-?1 in the denatured form as well as in the native form. Abs produced by HL1, HL3, and HL4 did not neutralize the induction of the MxA gene by IFN-?1. We also demonstrated the binding of the HL1 monoclonal anbitody and IFN-? complex on HepG2 cells.



Conclusion:Monoclonal Abs against IFN-?1 were produced. These Abs can be used to study the cellular binding and internalization of IFN-?.
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dc.language.isoen-
dc.titleGeneration and Characterization of Monoclonal Antibodies against Human Interferon-lambda1-
dc.typeArticle-
dc.identifier.urlhttp://www.ksimm.or.kr/journal/view.html?uid=1292&start=&sort=Regnum-0&scale=50&key=&oper=&key_word=&year1=&year2=&Vol=008&Num=01&PG=&book=Journal&mod=vol&sflag=&sub_box=Y&aut_box=Y&sos_box=&pub_box=Y&key_box=&abs_box=&year=2008-
dc.subject.keywordInterleukin 29 protein-
dc.subject.keywordmonoclonal antibody-
dc.contributor.affiliatedAuthor박, 선-
dc.type.localJournal Papers-
dc.citation.titleImmune network-
dc.citation.volume8-
dc.citation.number1-
dc.citation.date2008-
dc.citation.startPage7-
dc.citation.endPage12-
dc.identifier.bibliographicCitationImmune network, 8(1):7-12, 2008-
dc.identifier.eissn2092-6685-
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Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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