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Keto acid profiling analysis as ethoxime/tert-butyldimethylsilyl derivatives by gas chromatography-mass spectrometry.

Authors
Nguyen, DT; Lee, G; Paik, MJ
Citation
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 913-914:48-54, 2013
Journal Title
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN
1570-02321873-376X
Abstract
Organic acids, including keto acids, are key intermediates of central pathways in cellular metabolism. In this study, a comprehensive and reliable method was developed and optimized for the simultaneous measurement of 17 keto acids in various biological samples. The keto acids were converted to solvent extractable forms by ethoximation followed by tert-butyldimethylsilylation for direct analysis by gas chromatography-mass spectrometry in selected ion monitoring mode. The proposed method was precise (0.05-8.3, % RSD) and accurate (-10.5 to 5.3, % RE) with low limit of detection (0.01-0.5ng/mL) and good linearity (r>0.995) in the range of 0.01-5.0μg/mL. This was suitable for profiling analysis of targeted keto acids in human plasma, urine and rat brain tissue.
MeSH terms
AnimalsBrain ChemistryGas Chromatography-Mass Spectrometry/*methodsHumansHydrogen-Ion ConcentrationKeto Acids/*analysis/blood/chemistry/urineLimit of DetectionLinear ModelsOximes/*chemistryRatsReproducibility of ResultsSilanes/*chemistryTemperature
DOI
10.1016/j.jchromb.2012.11.021
PMID
23270938
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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