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Acidic metabolite profiling analysis of catecholamine and serotonin as O-ethoxycarbonyl/tert-butyldimethylsilyl derivatives by gas chromatography-mass spectrometry.

Authors
Nguyen, DT; Cho, IS; Kim, JW; Kim, KR; Lee, G; Paik, MJ
Citation
Biomedical chromatography : BMC, 27(2):216-221, 2013
Journal Title
Biomedical chromatography : BMC
ISSN
0269-38791099-0801
Abstract
A novel derivatization method was developed for the simultaneous determination of six acidic metabolites of catecholamine and serotonin by gas chromatography-mass spectrometry (GC-MS). The metabolites were converted to O-ethoxycarbonyl/tert-butyldimethylsilyl (EOC/TBDMS) derivatives for the direct GC-MS analysis in selected ion monitoring mode. Their mass spectral pattern as EOC/TBDMS derivatives showed characteristic fragment ions of [M - 15](+) and [M - 57](+), which permitted rapid and accurate structural confirmation of acidic metabolites. The present method was linear (r ≥ 0.998), reproducible (percentage relative standard deviation = 1.0-10.0) and accurate (% relative error = -9.7-9.8) with detection limits of 0.001-4.7 ng/mL. When applied to human urine samples, the method allowed simultaneous determination of six acidic metabolites of catecholamine and serotonin.
MeSH terms
AdultBiochemical ProcessesCatecholamines/chemistry/*urineGas Chromatography-Mass Spectrometry/*methodsHumansHydrogen-Ion ConcentrationLimit of DetectionLinear ModelsMaleOrganosilicon Compounds/*chemistryReproducibility of ResultsSerotonin/chemistry/*urineSilanes
DOI
10.1002/bmc.2778
PMID
22777873
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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