60 167

Cited 47 times in

MTERF1 binds mtDNA to prevent transcriptional interference at the light-strand promoter but is dispensable for rRNA gene transcription regulation.

Authors
Terzioglu, M; Ruzzenente, B; Harmel, J; Mourier, A; Jemt, E; López, MD; Kukat, C; Stewart, JB; Wibom, R; Meharg, C; Habermann, B; Falkenberg, M; Gustafsson, CM; Park, CB; Larsson, NG
Citation
Cell metabolism, 17(4):618-626, 2013
Journal Title
Cell metabolism
ISSN
1550-41311932-7420
Abstract
Mitochondrial transcription termination factor 1, MTERF1, has been reported to couple rRNA gene transcription initiation with termination and is therefore thought to be a key regulator of mammalian mitochondrial ribosome biogenesis. The prevailing model is based on a series of observations published over the last two decades, but no in vivo evidence exists to show that MTERF1 regulates transcription of the heavy-strand region of mtDNA containing the rRNA genes. Here, we demonstrate that knockout of Mterf1 in mice has no effect on mitochondrial rRNA levels or mitochondrial translation. Instead, loss of Mterf1 influences transcription initiation at the light-strand promoter, resulting in a decrease of de novo transcription manifested as reduced 7S RNA levels. Based on these observations, we suggest that MTERF1 does not regulate heavy-strand transcription, but rather acts to block transcription on the opposite strand of mtDNA to prevent transcription interference at the light-strand promoter.
MeSH terms
AnimalsCell LineDNA, Mitochondrial/genetics/*metabolismGene Expression RegulationMiceMice, KnockoutMitochondria/*genetics/metabolismMitochondrial Proteins/deficiency/genetics/*metabolismOxidative PhosphorylationPromoter Regions, GeneticProtein BindingRNA, Ribosomal/*metabolismRNA, Transfer/metabolismTranscription Factors/deficiency/genetics/*metabolismTranscription Initiation, Genetic
DOI
10.1016/j.cmet.2013.03.006
PMID
23562081
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
박, 찬배
Full Text Link
Files in This Item:
fulltext not available.txtDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse