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Isolation and characterization of a cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733.

Authors
Chae, HS; Lee, JM; Lee, JH; Lee, PC
Citation
Journal of microbiology and biotechnology, 23(6):837-842, 2013
Journal Title
Journal of microbiology and biotechnology
ISSN
1017-78251738-8872
Abstract
A cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733 was isolated, characterized, and used for the construction of a cloning vector to engineer Leuconostoc species. pMBLR00 is a rolling circle replication plasmid, containing 3,370 base pairs. Sequence analysis revealed that pMBLR00 has 3 open reading frames: Cop (copy number control protein), Rep (replication protein), and Mob (mobilization protein). pMBLR00 replicates by rolling circle replication, which was confirmed by the presence of a conserved double-stranded origin and single-stranded DNA intermediates. An Escherichia coli-Leuconostoc shuttle vector, pMBLR02, was constructed and was able to replicate in Leuconostoc citreum 95. pMBLR02 could be a useful genetic tool for metabolic engineering and the genetic study of Leuconostoc species.
MeSH terms
Bacterial Proteins/geneticsDNA Helicases/geneticsDNA ReplicationDNA, Bacterial/chemistry/geneticsEscherichia coli/geneticsGenes, BacterialGenetic VectorsGenetics, Microbial/methodsLeuconostoc/*geneticsMolecular Biology/methodsMolecular Sequence DataOpen Reading FramesPlasmids/*isolation & purificationSequence Analysis, DNATrans-Activators/genetics
PMID
23676906
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Journal Papers > School of Medicine / Graduate School of Medicine > Pediatrics & Adolescent Medicine
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