Cited 0 times in Scipus Cited Count

Protective effect of nicotinamide on high glucose/palmitate-induced glucolipotoxicity to INS-1 beta cells is attributed to its inhibitory activity to sirtuins.

DC Field Value Language
dc.contributor.authorLee, SJ-
dc.contributor.authorChoi, SE-
dc.contributor.authorJung, IR-
dc.contributor.authorLee, KW-
dc.contributor.authorKang, Y-
dc.date.accessioned2014-04-28T04:18:23Z-
dc.date.available2014-04-28T04:18:23Z-
dc.date.issued2013-
dc.identifier.issn0003-9861-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/9844-
dc.description.abstractThis study was initiated to determine whether the protective effect of nicotinamide (NAM) on high glucose/palmitate (HG/PA)-induced INS-1 beta cell death was due to its role as an anti-oxidant, nicotinamide dinucleotide (NAD+) precursor, or inhibitor of NAD+-consuming enzymes such as poly (ADP-ribose) polymerase (PARP) or sirtuins. All anti-oxidants tested were not protective against HG/PA-induced INS-1 cell death. Direct supplementation of NAD+ or indirect supplementation through NAD+ salvage or de novo pathway did not protect the death. Knockdown of the NAD+ salvage pathway enzymes such as nicotinamide phosphoribosyl transferase (NAMPT) or nicotinamide mononucleotide adenyltransferase (NMNAT) did not augment death. On the other hand, pharmacological inhibition or knockdown of PARP did not affect death. However, sirtinol as an inhibitor of NAD-dependant deacetylase or knockdown of SIRT3 or SIRT4 significantly reduced the HG/PA-induced death. These data suggest that protective effect of NAM on beta cell glucolipotoxicity is attributed to its inhibitory activity on sirtuins.-
dc.language.isoen-
dc.subject.MESHAcetylcysteine-
dc.subject.MESHAnimals-
dc.subject.MESHAntioxidants-
dc.subject.MESHCell Death-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Survival-
dc.subject.MESHEphrin-B2-
dc.subject.MESHGene Knockdown Techniques-
dc.subject.MESHGlucose-
dc.subject.MESHGlutathione-
dc.subject.MESHInsulin-Secreting Cells-
dc.subject.MESHMAP Kinase Kinase 4-
dc.subject.MESHNAD-
dc.subject.MESHNiacinamide-
dc.subject.MESHPalmitates-
dc.subject.MESHPhosphorylation-
dc.subject.MESHPoly Adenosine Diphosphate Ribose-
dc.subject.MESHProto-Oncogene Proteins c-akt-
dc.subject.MESHRats-
dc.subject.MESHSirtuin 3-
dc.subject.MESHSirtuins-
dc.subject.MESHTranscription Factor CHOP-
dc.titleProtective effect of nicotinamide on high glucose/palmitate-induced glucolipotoxicity to INS-1 beta cells is attributed to its inhibitory activity to sirtuins.-
dc.typeArticle-
dc.identifier.pmid23562377-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0003-9861(13)00099-4-
dc.contributor.affiliatedAuthor최, 성이-
dc.contributor.affiliatedAuthor이, 관우-
dc.contributor.affiliatedAuthor강, 엽-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.abb.2013.03.011-
dc.citation.titleArchives of biochemistry and biophysics-
dc.citation.volume535-
dc.citation.number2-
dc.citation.date2013-
dc.citation.startPage187-
dc.citation.endPage196-
dc.identifier.bibliographicCitationArchives of biochemistry and biophysics, 535(2). : 187-196, 2013-
dc.identifier.eissn1096-0384-
dc.relation.journalidJ000039861-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
Journal Papers > School of Medicine / Graduate School of Medicine > Endocrinology & Metabolism
Files in This Item:
There are no files associated with this item.

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse