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B-cell translocation gene 2: expression in the rat ovary and potential association with adenine nucleotide translocase 2 in mitochondria.

Authors
Park, JI; Kim, SG; Baek, MW; Park, TJ; Lim, IK; Seo, YW; Chun, SY
Citation
Molecular and cellular endocrinology, 367(1-2):31-40, 2013
Journal Title
Molecular and cellular endocrinology
ISSN
0303-72071872-8057
Abstract
The B-cell translocation gene 2 (Btg2) is an anti-proliferative tumor suppressor gene that behaves as a transcriptional regulator. The present study investigated gonadotropin induction of Btg2 in the rat ovary and the mechanism of Btg2 action as a partner of mitochondrial protein adenine nucleotide translocase 2 (Ant2). Transient induction of Btg2 as well as Btg1 mRNA levels by LH/hCG was observed in ovarian granulosa cells. Btg2 protein levels were also stimulated by LH/hCG. LH-induced gene expression of Btg2 required ERK signal pathway. Studies of deletion mutants in HeLa cells showed that deletion of Btg2 C-terminus (Btg2/ΔC) abolished the interaction with Ant2. In fact, the expression levels of Btg2/ΔC construct were decreased in mitochondrial fraction. Btg2 was also expressed in mitochondria and interacted with Ant2 in preovulatory granulosa cells. Interestingly, a Btg2/ΔC construct inhibited an action of Btg2 wild-type on ATP and H(2)O(2) production. These findings demonstrate the gonadotropin stimulation of Btg2 in the ovary and, the physical interaction of Btg2 with Ant2 in mitochondria.
MeSH terms
Adenine Nucleotide Translocator 2/genetics/*metabolismAdenosine Triphosphate/metabolismAnimalsChorionic Gonadotropin/pharmacologyFemaleGranulosa Cells/drug effects/metabolismHeLa CellsHumansHydrogen Peroxide/metabolismImmediate-Early Proteins/chemistry/genetics/*metabolismLuteinizing Hormone/pharmacologyMitochondria/drug effects/*enzymologyNeoplasm Proteins/genetics/metabolismOvary/drug effects/*metabolismProtein Binding/drug effectsProtein Transport/drug effectsRatsRats, Sprague-DawleySheepTumor Suppressor Proteins/chemistry/genetics/*metabolism
DOI
10.1016/j.mce.2012.12.013
PMID
23267836
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
박, 태준임, 인경
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