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TIS21/BTG2 inhibits invadopodia formation by downregulating reactive oxygen species level in MDA-MB-231 cells.

Authors
Choi, JA; Lim, IK
Citation
Journal of cancer research and clinical oncology, 139(10):1657-1665, 2013
Journal Title
Journal of cancer research and clinical oncology
ISSN
0171-52161432-1335
Abstract
PURPOSE: Invasion of cancer cells depends on the proteolytic degradation of extracellular matrix regulated by actin-driven membrane protrusions, called invadopodia. However, the mechanisms underlying invadopodia formation in cancer cells remain largely unknown.



METHODS: By employing adenoviral transduction of breast cancer cells with either β-galactosidase (Ad-LacZ) or TIS21(/BTG2/Pc3) (Ad-TIS21) gene, the regulation of invadopodia formation was investigated. Invasion activity was examined by invadopodia assay and Matrigel assay. Intracellular reactive oxygen species (ROS) was monitored by FACS-based analysis.



RESULTS: Here, we observed that TIS21 suppressed invadopodia formation as well as invasion activity along with F-actin remodeling. The inhibition of TIS21-mediated invadopodia formation was accompanied with attenuation of ROS generation in the TIS21 expressers, indicating that TIS21-mediated inhibition of ROS plays a critical role for invadopodia formation by regulating actin-associated protein remodeling. This was further confirmed in the TIS21(-/-)MEF cells.



CONCLUSIONS: This is the first report to provide insight into invasion signals regulated by tumor suppressor, TIS21(/BTG2/Pc3) gene, in the intractable breast cancer cells.
MeSH terms
Actin Cytoskeleton/metabolismAnimalsBreast Neoplasms/*pathologyCell Line, TumorCell Surface Extensions/*metabolismDown-RegulationFemaleFocal Adhesions/metabolismHumansImmediate-Early Proteins/*physiologyMiceMice, KnockoutNeoplasm InvasivenessReactive Oxygen Species/*metabolismTumor Suppressor Proteins/*physiology
DOI
10.1007/s00432-013-1484-3
PMID
23907596
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
최, 정아임, 인경
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