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Functional consequences of complementarity-determining region deactivation in a multifunctional anti-nucleic acid antibody.

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dc.contributor.authorLee, J-
dc.contributor.authorKim, HJ-
dc.contributor.authorRoh, J-
dc.contributor.authorSeo, Y-
dc.contributor.authorKim, M-
dc.contributor.authorJun, HR-
dc.contributor.authorPham, CD-
dc.contributor.authorKwon, MH-
dc.date.accessioned2014-05-08T05:18:11Z-
dc.date.available2014-05-08T05:18:11Z-
dc.date.issued2013-
dc.identifier.issn0021-9258-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/9908-
dc.description.abstractMany murine monoclonal anti-DNA antibodies (Abs) derived from mice models for systemic lupus erythematosus have additional cell-penetration and/or nucleic acid-hydrolysis properties. Here, we examined the influence of deactivating each complementarity-determining region (CDR) within a multifunctional anti-nucleic acid antibody (Ab) that possesses these activities, the catalytic 3D8 single chain variable fragment (scFv). CDR-deactivated 3D8 scFv variants were generated by replacing all of the amino acids within each CDR with Gly/Ser residues. The structure of 3D8 scFv accommodated single complete CDR deactivations. Different functional activities of 3D8 scFv were affected differently depending on which CDR was deactivated. The only exception was CDR1, located within the light chain (LCDR1); deactivation of LCDR1 abolished all of the functional activities of 3D8 scFv. A hybrid Ab, HW6/3D8L1, in which the LCDR1 from an unrelated Ab (HW6) was replaced with the LCDR1 from 3D8, acquired all activities associated with the 3D8 scFv. These results suggest that the activity of a multifunctional 3D8 scFv Ab can be modulated by single complete CDR deactivation and that the LCDR1 plays a crucial role in maintaining Ab properties. This study presents a new approach for determining the role of individual CDRs in multifunctional Abs with important implications for the future of Ab engineering.-
dc.language.isoen-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAmino Acid Substitution-
dc.subject.MESHComplementarity Determining Regions-
dc.subject.MESHDNA-
dc.subject.MESHHeLa Cells-
dc.subject.MESHHeparin-
dc.subject.MESHHumans-
dc.subject.MESHHydrolysis-
dc.subject.MESHImmunoglobulin Light Chains-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHProtein Structure, Secondary-
dc.subject.MESHRNA-
dc.subject.MESHSingle-Chain Antibodies-
dc.titleFunctional consequences of complementarity-determining region deactivation in a multifunctional anti-nucleic acid antibody.-
dc.typeArticle-
dc.identifier.pmid24155236-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3861637/-
dc.contributor.affiliatedAuthor권, 명희-
dc.type.localJournal Papers-
dc.identifier.doi10.1074/jbc.M113.508499-
dc.citation.titleThe Journal of biological chemistry-
dc.citation.volume288-
dc.citation.number50-
dc.citation.date2013-
dc.citation.startPage35877-
dc.citation.endPage35885-
dc.identifier.bibliographicCitationThe Journal of biological chemistry, 288(50). : 35877-35885, 2013-
dc.identifier.eissn1083-351X-
dc.relation.journalidJ000219258-
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Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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