169 346

Cited 31 times in

Genome-wide bimolecular fluorescence complementation analysis of SUMO interactome in yeast.

Authors
Sung, MK; Lim, G; Yi, DG; Chang, YJ; Yang, EB; Lee, K; Huh, WK
Citation
Genome research, 23(4):736-746, 2013
Journal Title
Genome research
ISSN
1088-90511549-5469
Abstract
The definition of protein-protein interactions (PPIs) in the natural cellular context is essential for properly understanding various biological processes. So far, however, most large-scale PPI analyses have not been performed in the natural cellular context. Here, we describe the construction of a Saccharomyces cerevisiae fusion library in which each endogenous gene is C-terminally tagged with the N-terminal fragment of Venus (VN) for a genome-wide bimolecular fluorescence complementation assay, a powerful technique for identifying PPIs in living cells. We illustrate the utility of the VN fusion library by systematically analyzing the interactome of the small ubiquitin-related modifier (SUMO) and provide previously unavailable information on the subcellular localization, types, and protease dependence of SUMO interactions. Our data set is highly complementary to the existing data sets and represents a useful resource for expanding the understanding of the physiological roles of SUMO. In addition, the VN fusion library provides a useful research tool that makes it feasible to systematically analyze PPIs in the natural cellular context.
MeSH terms
Cell Cycle/geneticsComputational Biology/methodsGene LibraryGene OrderGenetic Complementation Test*Genome-Wide Association Study/methodsProtein Binding*Protein Interaction Mapping/methodsReproducibility of ResultsSUMO-1 Protein/*genetics/*metabolismSaccharomyces cerevisiae/genetics/metabolismSaccharomyces cerevisiae Proteins/genetics/metabolismSubstrate SpecificityYeasts/*genetics/*metabolism
DOI
10.1101/gr.148346.112
PMID
23403034
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Medical Informatics
AJOU Authors
이, 기영
Full Text Link
Files in This Item:
23403034.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse