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Contribution of macrophages to enhanced regenerative capacity of dorsal root ganglia sensory neurons by conditioning injury.

Authors
Kwon, MJ; Kim, J; Shin, H; Jeong, SR; Kang, YM; Choi, JY; Hwang, DH; Kim, BG
Citation
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(38):15095-15108, 2013
Journal Title
The Journal of neuroscience : the official journal of the Society for Neuroscience
ISSN
0270-64741529-2401
Abstract
Although the central branches of the dorsal root ganglion (DRG) sensory neurons do not spontaneously regenerate, a conditioning peripheral injury can promote their regeneration. A potential role of macrophages in axonal regeneration was proposed, but it has not been critically addressed whether macrophages play an essential role in the conditioning injury model. After sciatic nerve injury (SNI) in rats, the number of macrophages in DRGs gradually increased by day 7. The increase persisted up to 28 d and was accompanied by upregulation of inflammatory mediators, including oncomodulin. A macrophage deactivator, minocycline, reduced the macrophage number and expressions of the inflammatory mediators. Molecular signatures of conditioning effects were abrogated by minocycline, and enhanced regenerative capacity was substantially attenuated both in vitro and in vivo. Delayed minocycline infusion abrogated the SNI-induced long-lasting heightened neurite outgrowth potential, indicating a role for macrophages in the maintenance of regenerative capacity. Intraganglionic cAMP injection also resulted in an increase in macrophages, and minocycline abolished the cAMP effect on neurite outgrowth. However, conditioned media (CM) from macrophages treated with cAMP did not exhibit neurite growth-promoting activity. In contrast, CM from neuron-macrophage cocultures treated with cAMP promoted neurite outgrowth greatly, highlighting a requirement for neuron-macrophage interactions for the induction of a proregenerative macrophage phenotype. The growth-promoting activity in the CM was profoundly attenuated by an oncomodulin neutralizing antibody. These results suggest that the neuron-macrophage interactions involved in eliciting a proregenerative phenotype in macrophages may be a novel target to induce long-lasting regenerative processes after axonal injuries in the CNS.
MeSH terms
Analysis of VarianceAnimalsAxons/physiologyCalcium-Binding Proteins/metabolismCell SeparationCells, CulturedCholera Toxin/metabolismCoculture TechniquesCyclic AMP/pharmacologyCytokines/metabolismDisease Models, AnimalDose-Response Relationship, DrugEnzyme-Linked Immunosorbent AssayFemaleFlow CytometryGAP-43 Protein/genetics/metabolismGanglia, Spinal/*pathologyGene Expression Regulation/drug effects/physiologyGlial Fibrillary Acidic ProteinMacrophages/drug effects/*physiologyMicrofilament Proteins/metabolismMinocycline/pharmacologyNerve Growth Factors/genetics/metabolismNerve Regeneration/*physiologyRatsRats, Sprague-DawleySciatic Neuropathy/*pathology/physiopathologySensory Receptor Cells/drug effects/*physiology
DOI
10.1523/JNEUROSCI.0278-13.2013
PMID
24048840
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Brain Science
AJOU Authors
황, 동훈김, 병곤
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