2026-09-22T19:12:07Zhttps://repository.ajou.ac.kr/oai/requestoai:repository.ajou.ac.kr:201003/118302024-10-11T02:21:23Zcom_201003_14372com_201003_14368col_201003_14373
Identification of Peptidyl-prolyl cis/trans isomerases affecting Drosophila circadian rhythms
초파리 생체리듬에 영향을 주는 프롤린 cis/trans 이성질화 효소의 규명
강, 소후
김, 은영
대학원 의생명과학과
201324124
강, 소후
ABSTRACT ⅰ
TABLE OF CONTENTS ⅲ
LIST OF FIGURES ⅴ
LIST OF TABLES ⅵ
Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 5
A. PPIase genes in Drosophila melanogaster 5
B. Knockdown fly strains 7
C. Fly behavioral assays 7
D. Plasmids and methods for S2 cell-based assays 8
E. Immunoblotting 8
F. Immunoprecipitation 9
G. Immunohistochemistry 10
H. Quantitative real-time RT–PCR 11
Ⅲ. RESULTS 12
A. PPIases alters Drosophila circadian activity rhythms 12
B. DOD 19
1. Daily cycle of core clock protein in dod knockdown flies 19
2. Degradatin of PER is attenuated when DOD is overexpressed in S2 cells 22
3. DOD interacts with hyperphosphorylated PER in S2 cells 28
C. CG5808 30
1. Daily cycle of core clock protein in CG5808 knockdown flies 30
2. PER and TIM are altered in key brain pacemaker neurons when CG5808 is down-regulated 34
Ⅳ. DISCCUTION 37
Ⅴ. CONCLUTION 40
REFERENCES 41
국문요약 46
Master
2015-10-28
2015-10-28
2015
2015
Thesis
Theses
http://repository.ajou.ac.kr/handle/201003/11830
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