2026-09-19T23:13:25Zhttps://repository.ajou.ac.kr/oai/request

oai:repository.ajou.ac.kr:201003/118372024-10-11T02:21:24Zcom_201003_14372com_201003_14368col_201003_14374

The effect of iron metabolism on palmitate-induced INS-1 cell death 고농도 지방산에 의한 INS-1 세포사에 철 대사가 미치는 영향 정, 익락 강, 엽 대학원 의생명과학과 110235 정, 익락 Apoptosis Endoplasmic reticulum(ER) stress Lipotoxicity Iron INS-1 Cell Palmitate Transferrin receptor Soduim fluorocitrate Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 24 A. MATERIALS 24 1. Reagents 24 B. METHODS 25 1. Cell Culture 25 2. Preparation of palmitate 25 3. Isolation of islets 25 4. MTT-bases viability assay 26 5. Measurement of cell death 26 6. Western blot analysis 27 7. RNA extraction and microarray analysis 27 8. Reverse transcriptase-polymerase chain reaction and quantitative PCR 28 9. DNA and RNA Transfection 28 10. Measurement of intracellular labile iron pool (LIP) 29 11. Aconitase activity assay 29 12. Oxygen consumption rate (OCR) 30 13. PA uptake 31 14. Quantitation of insulin 31 15. Immunocytochemistry 32 16. Statistics 32 Ⅲ. RESULTS [Part I] 36 A. Palmitate treatment reduces intracellular iron level in INS-1 beta cell. 36 B. Iron depletion and supplementation in palmitate-induced INS-1 cell death. 41 C. Toxic mediator of iron deficiency. 50 D. Beta-cell function by iron regulation. 60 E. Effect of iron regulation on lipotoxicity in other cells. 61 Ⅲ. RESULTS [Part II] 64 A. Control of PA-induced INS-1 cell death through the TCA cycle intermediate. 64 B. Role of SFA and SFC in various of cell death models. 71 C. The effect of aconitase inhibition on PA-induced lipotoxicity 76 D. SFC altered iron-regulating proteins. 84 E. Changes in intracellular signaling associated with SFC treatment. 88 F. Changes in oxygen consumption rate associated with SFC treatment. 98 G. SFC modulates CD36 translocation and reduces PA uptake. 100 H. SFA and SFC protect against PA-induced islet cell death. 103 Ⅳ. DISCUSSION [Part I] 104 Ⅳ. DISCUSSION [Part II] 110 Ⅴ. CONCLUSION 117 REFERENCES 118 국문요약 135 Doctor 2015-10-28T03:10:47Z 2015-10-28T03:10:47Z 2015 2015 Thesis Theses http://repository.ajou.ac.kr/handle/201003/11837 http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020143 000000020143 en application/pdf