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oai:repository.ajou.ac.kr:201003/118922024-10-11T02:21:24Zcom_201003_14369com_201003_14368col_201003_14371

Chronic Ocular Hypertensive Model using Microbead Injection in Rats: Comparison of Polyurethane, Polymethylmethacrylate, and Polystyrene 쥐에서 미세구슬주입술을 이용한 만성 고안압증 모델; 폴리우레탄과 폴리메틸메타크릴레이트, 폴리스티렌재질의 비교 노, 승수 안, 재홍 대학원 의학과 200524171 노, 승수 Chronic ocular hypertension Microbead Axon Retinal ganglion cell Intraocular pressure Polyurethane 만성 고안압증 미세구슬 축삭돌기 망막신경절세포 안압 폴리우레탄 Purpose: To establish and assess an ocular hypertensive rat model using intracameral injection with various microbeads of different sizes and materials. Methods: Chronic elevation of intraocular pressure (IOP) was induced by injection of various microbeads into the anterior chamber of Sprague-Dawley rat eyes. We compared the IOPs induced by the injection of different microbeads [7- and 17-µm polyurethane (PU), 7- and 15-µm polymethylmethacrylate (PMMA), and 15-µm polystyrene (PS)] and selected the appropriate microbeads for a chronic ocular hypertensive model in terms of IOP elevation and adverse events. IOP changes were observed for 4 weeks after microbead injections. Axonal degeneration was assessed with transmission electron microscopic photographs and RGC loss was assessed with retrograde labeling. Results: Sixty-nine rats were included. Three days after a single injection of microbeads, IOPs were increased by 24.0% by 7-µm PU microbeads, 101.8% by 17-µm PU microbeads, 56.6% by 7-µm PMMA microbeads, 22.0% by 15-µm PMMA microbeads, and 34.7% by 15- µm PS microbeads. 17-µm PU microbeads produced constant IOP elevation with good reproducibility (standard deviation of < 6.6 mmHg). Sustained IOP elevation by two injections of 17-µm PU microbeads resulted in a 42% axon loss and 36.5% RGC loss (p<0.05, Mann-Whitney U test). Conclusions: PU microbead injections offer an applicable and versatile model for a chronic ocular hypertensive model in rats. Among several biomaterials, PU microbeads produced a more stable IOP elevation without adverse events. I.INTRODUCTION 1 II. MATERIALS AND METHODS 3 A. Animal use 3 B. IOP measurement 3 C. Microbead injections 3 D. Tissue preparation 5 E. Quantification of axonal loss 6 F. Retrograde labeling and quantification of RGCs 8 G. Statistical analysis 9 III. RESULTS 11 A. IOP Elevation with Microbead Injections11 B. IOP Elevation with Repeated PU Microbead Injections 12 C. Measurement of Adverse Effects after Microbead Injection 17 D. Comparison of Axon Damage 19 E. Confirmation of accuracy of semi-automated axonal count 19 F. RGC damage assessed by retrograde labeling 21 IV. DISCUSSION 33 V. CONCLUSION 37 REFERENCES 38 국문요약 42 Doctor 2015-11-05T03:25:40Z 2015-11-05T03:25:40Z 2015 2015 Thesis Theses http://repository.ajou.ac.kr/handle/201003/11892 http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020213 000000020213 en application/pdf