2026-09-22T07:00:34Zhttps://repository.ajou.ac.kr/oai/request

oai:repository.ajou.ac.kr:201003/119032024-10-11T02:21:24Zcom_201003_14369com_201003_14368col_201003_14371

The effect of cutaneous vasculature on skin pigmentation 피부 혈관이 피부 색소변화에 끼치는 영향 박, 지윤 강, 희영 대학원 의학과 107328 박, 지윤 Co-culture Cutaneous vasculature Endothelial cells Hyperpigmentary disorder Melanocytes Melasma Skin pigmentation ABSTARCT i TABLE OF CONTENTS ii LIST OF FIGURES iv LIST OF TABLES vii I. INTRODUCTION 1 II. MATERIALS AND METHODS 3 A. Cell cultures and treatment 3 B. Co-culture using transwell system 4 C. Preparation of the bEnd.3/HUVECs conditioned medium 4 D. Ultraviolet (UV) irradiation of endothelial cells 5 E. Endothelial tube formation assay model using GeltrexTM Matrix 5 F. Hypoxia treatments 5 G. Melanin content and tyrosinase activity 5 H. Western blot analysis 6 I. Real-time PCR and RT-PCR 6 J. RNA sequence analysis 7 K. Ex vivo skin organ culture and pigmentation assay of cultured skin 7 L. Statistical analysis 8 III. RESULTS 9 A. Preliminary study 9 1. Effect of HUVECs on the proliferation of fibroblasts 9 B. The presence of endothelial cells has a negative effect on pigmentation 10 1. The effect of murine endothelial cells on pigmentation 10 2. The verification of the effect of endothelial cells with human cells 12 C. Additional modification of endothelial cells 15 1. UV irradiation: Contrary to non-stimulated cells, UV-irradiated endothelial cells induce pigmentation 15 2. VEGF treatment 19 3. Endothelial tube formation assay using GeltrexTM Matrix 20 4. Endothelial cell-conditioned media with hypoxic condition 22 5. Triple co-culture study with NHMs, HUVECs, and fibroblasts 23 D. Identification of possible melanogenic factors secreted by endothelial cells 24 1. Identification of endothelin-1 as one of melanogenic factors secreted by UV-irradiated endothelial cells 24 2. Identification of candidate cytokines derived from HUVECs using angiogenesis-related cytokine antibody array 28 IV. DISCUSSION 31 V. CONCLUSION 36 REFERENCES 37 국문요약 44 Doctor 2015-11-05T06:37:45Z 2015-11-05T06:37:45Z 2015 2015 Thesis Theses http://repository.ajou.ac.kr/handle/201003/11903 http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020277 000000020277 en application/pdf