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박, 해심 김, 성호 대학원 의학과 200624447 김, 성호 2011-01-18T01:53:42Z 2011-01-18T01:53:42Z 2010 2010 http://repository.ajou.ac.kr/handle/201003/1196 http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000010909 000000010909 Approved for entry into archive by Hyun Chul Cho (hccho@ajou.ac.kr) on 2011-01-18T01:53:42Z (GMT) No. of bitstreams: 1 000000010909.pdf: 1754328 bytes, checksum: f368ea5788d938b5f460cda2310348ec (MD5) Made available in DSpace on 2011-01-18T01:53:42Z (GMT). No. of bitstreams: 1 000000010909.pdf: 1754328 bytes, checksum: f368ea5788d938b5f460cda2310348ec (MD5) Submitted by Hyun Chul Cho (hccho@ajou.ac.kr) on 2011-01-18T01:53:42Z No. of bitstreams: 1 000000010909.pdf: 1754328 bytes, checksum: f368ea5788d938b5f460cda2310348ec (MD5) ABSTRACT .i TABLE OF CONTENTS v LIST OF FIGURES viii ABBREVIATIONS x I.INTRODUCTION 1 II. MATERIALS AND METHODS 7 A. Method 7 1. Epithelial cell culture and tTG activity assay 7 2. Intracellular reactive oxygen species (ROS) measurement 8 3. Immunoblot analysis 8 4. In vitro cross-linking reaction assay 9 5. Statistical analysis 9 B. Method 10 1. Materials 10 2. Cell culture 10 3. Real-Time polymerase chain reaction 10 4. Preparation of whole, cytosolic and nuclear extracts 11 5. Western blot analysis 12 6. Electrophoretic Mobility Shift Assay (EMSA) 13 7. Immunocytochemistry 13 8. Statistical analysis 14 C. Method 14 1. Cells culture and treatment with TDI 14 2. Real-time reverse transcriptase polymerase chain reaction 15 3. Preparation of FITC-DBP conjugate and immunofluorescence staining 16 4.Flow cytometry analysis 16 5. Western blot analysis 17 6. Measurement of 1,25(OH)2D3 production and VEGF secretion 17 7. Statistical Analyses 18 III. RESULTS 19 A. Results 19 1. TDI-HSA conjugate increased tTG activity in A549 cells via ROS production 19 2. The cross linking between tTG and CK19 26 B. Results 29 1. TDI down-regulated FTL expression in A549 cells 29 2. TDI down-regulated HO-1 expression in A549 cells 31 3. TDI inhibited Nrf2 translocation into nucleus 35 4. TDI inhibited the activation of MAP kinases 38 5. PPARγ agonists rescued the effect of TDI on the expression of HO-1/FTL 41 C. Results 44 1. Suppresses of the uptake of FITC-VDBP into RLE-6TN cells by TDI 44 2. Megalin mediated the endocytic uptake of FITC-VDBP into RLE-6TN cells 46 3. Downregulation of megalin mRNA and protein by TDI treatment in RLE-6TN 49 4. Decreased 1,25(OH)2D3 production by TDI 51 5. Increased VEGF production and secretion through suppression of the 1,25(OH)2D3 production 52 IV. DISCUSSION 55 V. CONCLUSION 67 REFERENCES 68 -국문요약- 85 Doctor en TDI 천식 기도염증반응과 관련된 활성산소의 역할 Thesis Theses 2010 9999-12-31 9999-12-31