2026-09-23T13:14:58Zhttps://repository.ajou.ac.kr/oai/request

oai:repository.ajou.ac.kr:201003/130372024-10-11T02:21:25Zcom_201003_14372com_201003_14368col_201003_14374

Expression and regulation of genes associated with CNS regeneration and plasticity 중추신경계 재생 및 가소성에 관여하는 유전자 발현 및 조절 신, 혜영 김, 병곤 대학원 의생명과학과 201024494 신, 혜영 Axon regeneration conditioning injury RAGs epigenetics I. INTRODUCTION 1 A. Spinal cord injury pathophysiology 1 B. Factors for the failure of CNS axon regeneration 2 C. Conditioning injury (CI)-induced axon regeneration 3 D. Regeneration associated genes (RAGs) 4 E. Epigenetics 5 F. Neuroepigenetics in CNS injury 8 G. Next generation sequencing (NGS) in epigenomic studies 10 H. Aims of this study 11 II. Materials and methods 13 1. Animals and surgical procedures 13 2. In vivo 5-aza and SAM administration 13 3. Primary culture of dissociated adult DRG neurons 14 4. Genomic DNA extraction for MeDIP-seq 14 5. Methylated DNA immunoprecipitation and sequencing 15 6. Bioinformatics analysis 15 7. Bisulfite sequencing 17 8. Chromatin immunoprecipitation assay 18 9. ChIP-PCR primers 19 10. qRT-PCR primers 20 11. Methylation assay 21 12. Tissue processing and immunohistochemistry 21 13. Quantification 22 14. Production of recombinant adeno-associated virus (AAV) 23 15. Statistical Analysis 23 III. RESULTS 25 Part A. Changes in DNA methylation landscape in DRG sensory neurons after conditioning injury 25 1. Changes in global DNA methylation landscape following conditioning injury 25 2. Correlation between DNA methylation and RNA transcription 34 3. Functional significance of DNA methylation 37 Part B. Demethylation of c-Myc gene triggers transcription of a group of RAGs in DRG sensory neurons following conditioning injury 42 1. Transcription factor gene selection using Oreganno-analysis 42 2. c-Myc expression is upregulated in DRGs in response to CI 44 3. The effects of c-Myc overexpression by AAV5 without CI 49 4. Histone modifications involvement in the regulation of c-Myc after CI 57 IV. DISCUSSION 60 V. SUMMARY AND CONCLUSION 68 REFERENCES 69 국문요약 85 Doctor 2016-11-29T05:41:06Z 2016-11-29T05:41:06Z 2016 2016 Thesis Theses http://repository.ajou.ac.kr/handle/201003/13037 http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000021963 000000021963 en application/pdf