2026-09-23T04:49:51Zhttps://repository.ajou.ac.kr/oai/request

oai:repository.ajou.ac.kr:201003/14002024-10-11T02:17:36Zcom_201003_14369com_201003_14368col_201003_14370

김, 철호 한, 남수 대학원 의학과 200724417 한, 남수 2011-01-31T07:12:48Z 2011-01-31T07:12:48Z 2009 2009 http://repository.ajou.ac.kr/handle/201003/1400 http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009815 000000009815 "Expression and Clinical Significance of The N-myc downstream regulated gene-1 in Hypopharyngeal Cancer Objective. N-myc downstream regulated gene-1 (Ndrg-1), a potent metastasis suppressor gene, is identified as a gene that is up-regulated by the induction of differentiation in various cancer cell lines. Ndrg-1 has been shown to be involved in tumor progression and metastasis in colon, prostate and breast cancer. However, the clinical significance of Ndrg-1 in hypopharyngeal cancer remains to be investigated, and we therefore examined the role of Ndrg-1 in the survival rate, invasion and metastasis of hypopharyngeal squamous cell carcinoma (SCC). Materials and methods. We performed immunohistochemical staining of 56 specimens each of normal mucosa and SCC of the hypopharynx with Ndrg-1 antibodies. For reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting, fresh normal and cancer tissue from the hypopharynx obtained from three patients were used. Results. Positive rates of Ndrg-1 expression in hypopharyngeal SCC were 21.4%, respectively. Ndrg-1 staining was significantly inverse correlated with lymph node metastasis. (p=0.048) Decreased expression of Ndrg-1 protein (Western blotting) was detected in hypopharyngeal cancer tissue. Conclusion. Our results suggest that N-myc downstream regulated gene-1 (Ndrg-1) may play an important role in the progression and metastasis of hypopharyngeal cancer. " Approved for entry into archive by Hyun Chul Cho (hccho@ajou.ac.kr) on 2011-01-31T07:12:48Z (GMT) No. of bitstreams: 1 000000009815.pdf: 15735691 bytes, checksum: 617e331999e3546a19faf4d10b06dd5d (MD5) Made available in DSpace on 2011-01-31T07:12:48Z (GMT). No. of bitstreams: 1 000000009815.pdf: 15735691 bytes, checksum: 617e331999e3546a19faf4d10b06dd5d (MD5) Submitted by Hyun Chul Cho (hccho@ajou.ac.kr) on 2011-01-31T07:12:48Z No. of bitstreams: 1 000000009815.pdf: 15735691 bytes, checksum: 617e331999e3546a19faf4d10b06dd5d (MD5) "TABLE OF CONTENTS ABSTRACT i TABLE OF CONTENTS ii LIST OF FIGURES iii LIST OF TABLES iv Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 3 A. Patients 3 B. Immunohistochemistry 3 C. Reverse transcriptase polymerase chain reaction 4 D. Western blotting 5 Ⅲ. RESULTS 6 A. Expression of Ndrg-1 in hypopharyngeal cancer tissue 6 B. RT-PCR and Western blotting in hypopharyngeal cancer tissue 9 Ⅳ. DISCUSSION 11 Ⅴ. CONCLUSION 16 REFERENCES 17 국문요약 21 " Master application/pdf en Expression and Clinical Significance of the N-myc downstream regulated gene-1 in Hypopharyngeal Cancer Ndrg-1의 하인두암에서의 발현과 임상적 의의 Thesis Theses Hypopharyngeal clinical Ndrg-1 expression 2009 9999-12-31 9999-12-31