2026-09-22T03:00:59Zhttps://repository.ajou.ac.kr/oai/requestoai:repository.ajou.ac.kr:201003/22422024-10-11T02:17:37Zcom_201003_14372com_201003_14368col_201003_14373
Modulation of Brain Inflammation Induced by Interaction between Astrocytes, Neutrophils and Monocytes
TRAN, AN
조, 은혜
대학원 의생명과학과
200824382
TRAN, AN
brain inflammation
astrocyte
monocyte, microglia
TABLE OF CONTENTS
ABSTRACT i
TABLE OF CONTENTS ii
LIST OF FIGURES iv
LIST OF TABLES v
ABBREVIATIONS vi
I. Introduction 1
A. Brain inflammation 1
B. Microglia and astrocytes as main regulators of brain inflammation 1
1. Microglia 1
2. Astrocytes 3
C. Leukocytes: immune cells to infiltrate the injured brain 3
D. Regulation of brain inflammation 5
1. Stimulators of microglia 5
2. Proinflammatory cytokines 6
3. Chemokines 7
4. Growth factors 9
E. Aims of this study 10
II. MATERIALS AND METHODS 11
1 Cell culture 11
2. Reverse Transcription and Polymerase Chain Reaction (RT-PCR) 12
3. Western blot analysis 12
4. Immunocytochemistry 13
5. Preparation of conditioned media 13
6. ELISA (Enzyme-linked immunosorbent assay) 13
7. Migration assay 14
8. Statistical analysis 14
III. RESULTS 16
A. Interaction between astrocytes and neutrophils 16
1. Effect of neutrophils on morphology of astrocytes 16
2. Effect of neutrophils on inflammatory responses of astrocytes 18
B. Interaction between astrocytes and monocytes 20
1. Effect of THP1 cells on morphology of astrocytes 20
2. Effect of THP1 cells on inflammatory responses of astrocytes 24
3. Migration assay 26
4. Dose-dependent effect of TCM 29
5. Astrocytes regulate inflammatory responses in THP1 cells 29
IV. DISCUSSION 32
V. CONCLUSION 35
REFERENCES 36
Master
2011-04-13T04:46:34Z
2011-04-13T04:46:34Z
2010
2010
Thesis
Theses
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