2026-09-24T05:44:06Zhttps://repository.ajou.ac.kr/oai/request

oai:repository.ajou.ac.kr:201003/22542024-10-11T02:17:36Zcom_201003_14372com_201003_14368col_201003_14373

단순포진바이러스로 유도된 베체트병 마우스모델에서 CD4+CD25+ 조절 T 세포의 역할 심, 주아 손, 성향 대학원 의생명과학과 200724296 심, 주아 베체트병 "ABSTRACT TABLE OF CONTENTS LIST OF FIGURE LIST OF TABLES Ⅰ. INTRODUCTION Ⅱ. MATERIALS AND METHODS A. Antibodies and reagents B. Animal experiments C. BD-like symptoms D. Flow cytometry E. Splenocyte culture and generation of Treg cells F. Dendritic cells isolation and culture G. Co-culture of CD4+T cells with DCs H. Isolation of CD4+CD25+T cells I.Adoptive transfer of CD4+CD25+T and CD4+CD25-T cells to BD-like mice J. ELISA K. Reverse transcription PCR (RT-PCR) L. Statistical analysis Ⅲ. RESULTS A. CD4+CD25+ Treg cells in BD-like mice compared to BDN mice B. CD4+CD25+T cells were amplified in primary cultures of spleen tissues C. CD4+T cells from normal, BDN, and BD-like splenocytes were co-cultured with normal dendritic cells from normal mice D. Adoptive transfer with CD4+CD25+T cells up-regulated the frequencies of T cell in BD-like mice E. Change of symptoms in BD-like mice after adoptive transfer F. The change of severity score in transferred with CD4+CD25+T cells to BD-like mice G. CD4+CD25+T cells transfer up-regulated serum IL-10 and TGF-β levels and down-regulated IFN-γ and TNF-α levels H. Serum IL-6 and IL-17 levels were down-regulated after transfer I. The differences of serum cytokine amounts between the average of not transferred BD-like mice and 3x10^5 CD4+CD25+T cells transferred each BD-like mouse Ⅳ. DISCUSSION Ⅴ. CONCLUSION REFERENCES 국문요약 " Master 2011-04-13T05:07:06Z 2011-04-13T05:07:06Z 2009 2009 Thesis Theses http://repository.ajou.ac.kr/handle/201003/2254 http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009672 000000009672 ko