2026-09-22T22:44:33Zhttps://repository.ajou.ac.kr/oai/request

oai:repository.ajou.ac.kr:201003/75392024-10-11T02:17:40Zcom_201003_14372com_201003_14368col_201003_14373

Anti-inflammatory Role of IL-15Rα Up-regulated by Poly I:C in Herpes Simplex Virus-induced Behcet's Disease-like Mouse Model 단순포진 바이러스로 유도한 베체트병 마우스모델에서 Poly I:C 에 의해 유도된 IL-15Rα의 항 염증 효과에 관한 연구 최, 주영 손, 성향 대학원 의생명과학과 201024353 최, 주영 Behcet's disease IL-15Rα Poly I:C memory CD8+ T cells Herpes Simplex virus mouse model Behcet’s disease (BD) is a chronic, multi-systemic disorder that has arthritic, intestinal, mucocutaneous, ocular, vascular, and central nervous system affects. Interleukin-15 receptor alpha (IL-15Rα) forms stable complex with IL-15 on the cell surface of activated monocytes and mediates the proliferation of memory CD8+ T cells. Recent studies informed that Polyinosinic:polycytidylic acid (Poly I:C) is an immunostimulant which boosts the generation of memory T cells though induction of IL-15 and IL-15Rα. We found that the frequencies of IL-15Rα were significantly lower in HSV-induced BD mouse model than control mice. Therefore, this study was conducted to determine whether Poly I:C supplementation could induce memory cells through up-regulation of IL-15Rα, and reduce inflammation in HSV-induced BD symptoms. To evaluate whether the expression of IL-15Rα could be increased by poly I:C in vivo, Poly I:C was administered into normal and BD mice. Then, Poly I:C administered BD mice were subsequently observed the change of symptoms until 17 days after the last injection. The frequencies of IL-15Rα and memory CD8+ T cells were significantly up-regulated in Poly I:C injected mice. Poly I:C improved the symptoms and decreased severity score through down-regulation of pro-inflammatory cytokines and elevated regulatory and memory T cells. In conclusion, these results suggest that IL-15Rα is an important factor for the regulation of symptoms in HSV-induced BD mice. TABLE OF CONTENTS ABSTRACT ⅰ TABLE OF CONTENTS Ⅲ LIST OF FIGURE ⅴ LIST OF TABLES ⅶ Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 3 A. Animal experiments 3 B. BD symptoms in a mouse model 3 C. In vivo administration of Poly I:C 4 D. Flow cytometric analysis of cell surface staining 4 E. ELISA 5 F. Reverse transcription PCR (RT-PCR) 5 G. Statistical analysis 6 Ⅲ. RESULTS 7 A. The frequencies of IL-15Rα expressing cells in BD mice compared with BDN mice 9 B. Poly I:C up-regulates expression of IL-15Rα in PBMC of normal mice 12 C. Poly I:C ameliorates HSV-induced BD symptoms 16 D. Poly I:C up-regulates the frequencies of IL-15Rα but not IL-7Rα (CD127) in BD mice 18 E. Poly I:C induces CD8+CD44+ memory T cells in BD mice 20 F. Poly I:C up-regulate CD122(IL-15/2Rβ) and CD8+CD122+ T cells in BD mice 24 G. Poly I:C down-regulates IL-23 receptor and IL-17A in BD mice 28 Ⅳ. DISCUSSION 30 Ⅴ. CONCLUSION 34 REFERENCES 36 국문요약 45 Master 2012-10-26T03:54:39Z 2012-10-26T03:54:39Z 2012 2012 Thesis Theses http://repository.ajou.ac.kr/handle/201003/7539 http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000012284 000000012284 en