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Epicatechin protects auditory cells against cisplatin-induced death.

Authors
Kim, CH; Kang, SU; Pyun, J; Lee, MH; Hwang, HS; Lee, H
Citation
Apoptosis : an international journal on programmed cell death, 13(9):1184-1194, 2008
Journal Title
Apoptosis : an international journal on programmed cell death
ISSN
1360-81851573-675X
Abstract
Cisplatin, a chemotherapeutic drug that is widely used to treat various cancers, promotes ototoxicity at higher doses. In this study, the effect of epicatechin (EC) on cisplatin-induced hair cell death was investigated in a cochlear organ of Corti-derived cell line, HEI-OC1, and in vivo in zebrafish. Cisplatin promoted apoptosis and altered mitochondrial membrane potential (MMP) in HEI-OC1 cells. EC inhibited cisplatin-induced apoptosis and intracellular reactive oxygen species (ROS) generation. Labeling of zebrafish lateral line hair cells by the fluorescent dye YO-PRO1 was lost upon exposure to cisplatin, and EC protected against this cisplatin-induced loss of labeling in a dose-dependent manner. Scanning and transmission electron micrographs showed that treatment with EC protected against cisplatin-induced loss of kinocilium and stereocilia in zebrafish neuromasts. These results suggest that EC prevents cisplatin-induced ototoxicity by blocking ROS generation and by preventing changes in MMP.
MeSH terms
AnimalsCaspase 3/metabolismCatechin/chemistryCatechin/pharmacology*Cell Death/drug effectsCell LineCell Survival/drug effectsCisplatin/antagonists & inhibitorsCisplatin/pharmacology*Cytoprotection/drug effects*Hair Cells, Auditory/cytology*Hair Cells, Auditory/drug effects*Hair Cells, Auditory/enzymologyHair Cells, Auditory/ultrastructureMembrane Potential, Mitochondrial/drug effectsReactive Oxygen Species/metabolismZebrafish
DOI
10.1007/s10495-008-0242-5
PMID
18670884
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
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