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Simultaneous clinical monitoring of lactic acid, pyruvic acid and ketone bodies in plasma as methoxime/tert-butyldimethylsilyl derivatives by gas chromatography-mass spectrometry in selected ion monitoring mode.

Authors
Paik, MJ; Cho, EY; Kim, H; Kim, KR; Choi, S; Ahn, YH; Lee, G
Citation
Biomedical chromatography : BMC, 22(5):450-453, 2008
Journal Title
Biomedical chromatography : BMC
ISSN
0269-38791099-0801
Abstract
Simultaneous determination of lactic acid, pyruvic acid, 3-hydroxybutyric acid and acetoacetic acid for clinical monitoring of lactic acidosis and ketone body formation in human plasma (20 microL) was performed by gas chromatography-mass spectrometry in selected ion monitoring (SIM) mode after generating methoxime/tert-butyldimethylsilyl derivatives. All of the targeted carboxylic acids were detected by characteristic fragment ions, which permitted sensitive and selective identification in the presence of co-extracted free fatty acids and other acidic metabolites at much higher levels. The method was linear (r>or=0.9991), reproducible (% relative standard deviation=1.2-5.8), and accurate (% relative error=-7.2-7.6), with detection limits of 0.05-1.7 ng/mL. This rapid, accurate and selective method using minimal plasma samples (20 microL) is useful in the clinical monitoring of lactic acidosis and ketone body formation in plasma.
MeSH terms
Gas Chromatography-Mass Spectrometry/methods*HumansKetone Bodies/blood*Ketone Bodies/chemistryLactic Acid/blood*Lactic Acid/chemistryMaleOrganosilicon Compounds/chemistry*Oximes/chemistry*Pyruvic Acid/blood*Pyruvic Acid/chemistryReproducibility of Results
DOI
10.1002/bmc.966
PMID
18254151
Appears in Collections:
Journal Papers > Research Organization > Institute for Neuroregeneration & Stem Cell Research
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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