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Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice.

Authors
Krishnan, J; Chen, J; Shin, KJ; Hwang, JI; Han, SU; Lee, G; Choi, S
Citation
Experimental & molecular medicine, 40(5):495-504, 2008
Journal Title
Experimental & molecular medicine
ISSN
1226-36132092-6413
Abstract
Exposure to light can induce photoreceptor cell death and exacerbate retinal degeneration. In this study, mice with genetic knockout of several genes, including rhodopsin kinase (Rhok-/-), arrestin (Sag-/-), transducin (Gnat1-/-), c-Fos (c-Fos-/-) and arrestin/transducin (Sag-/-/Gnat1-/-), were examined. We measured the expression levels of thousands of genes in order to investigate their roles in phototransduction signaling in light-induced retinal degeneration using DNA microarray technology and then further explored the gene network using pathway analysis tools. Several cascades of gene components were induced or inhibited as a result of corresponding gene knockout under specific light conditions. Transducin deletion blocked the apoptotic signaling induced by exposure to low light conditions, and it did not require c-Fos/AP-1. Deletion of c-Fos blocked the apoptotic signaling induced by exposure to high intensity light. In the present study, we identified many gene transcripts that are essential for the initiation of light-induced rod degeneration and proposed several important networks that are involved in pro- and anti-apoptotic signaling. We also demonstrated the different cascades of gene components that participate in apoptotic signaling under specific light conditions.
MeSH terms
AnimalsApoptosis/radiation effectsG-Protein-Coupled Receptor Kinase 1/geneticsGTP-Binding Protein alpha Subunits/geneticsGene Expression Profiling*Genes, fos/geneticsLight/adverse effectsLight Signal Transduction/genetics*Light Signal Transduction/physiologyLight Signal Transduction/radiation effectsMiceMice, KnockoutOligonucleotide Array Sequence AnalysisRetina/metabolismRetina/pathologyRetina/radiation effectsRetinal Degeneration/etiologyRetinal Degeneration/genetics*Retinal Degeneration/physiopathologyTransducin/genetics
DOI
10.3858/emm.2008.40.5.495
PMID
18985007
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Surgery
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
AJOU Authors
한상욱이광
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