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Alleviation of osteoarthritis by calycosin-7-O-beta-D-glucopyranoside (CG) isolated from Astragali radix (AR) in rabbit osteoarthritis (OA) model.

Authors
Choi, SI; Heo, TR; Min, BH; Cui, JH; Choi, BH; Park, SR
Citation
Osteoarthritis and cartilage, 15(9):1086-1092, 2007
Journal Title
Osteoarthritis and cartilage
ISSN
1063-45841522-9653
Abstract
OBJECTIVE: This work was undertaken to assess the protective effect of an isoflavonoid, calycosin-7-O-beta-D-glucopyranoside (CG), isolated from Astragali radix (AR) on the pathogenesis of osteoarthritis (OA)-like lesion in a rabbit model.



METHODS: Nine rabbits underwent an anterior cruciate ligament and menisectomy transection (ACLMT) of the rear knee joints to induce OA-like lesion. They were randomly divided into three groups (n=6/group): a negative control group treated with 200 microl of 0.5% (v/v) dimethyl sulfoxide (DMSO), a positive control group treated with 200 microl of 100 microM piroxicam, and a test group treated with 100 microg/500 microl of CG, where the test agents were administered by injection once a week for 4 weeks starting from the third week. Rabbits were then sacrificed to observe the progression of OA-like lesion. The synovial fluid was analyzed for the amounts of total proteins, glycosaminoglycans (GAG) and prostaglandin E(2) (PGE(2)). In addition, histopathologic analyses were performed on the OA-like articular cartilage with or without therapeutic treatments.



RESULTS: The total synovial fluid volume (P<0.05) was most strikingly reduced by the treatment with CG. Moreover, the CG treatment also significantly alleviated the OA-induced accumulation of prostaglandin (PG) (P<0.001) and total proteins (P<0.001) in the synovial fluid. The histopathologic analyses revealed that the CG treatment reduced the severity of the OA-like structural damages in the cartilage. However, the level of PGE(2), a pathologic inflammatory molecule, was not diminished by CG or piroxicam.



CONCLUSION: These results indicate that the isoflavonoid CG isolated from AR significantly alleviated the pathologic changes in the OA-like rabbit knee joints. This suggests that CG from AR could be a promising treatment for the therapy of OA.
MeSH terms
AnimalsAnterior Cruciate Ligament/surgeryAnti-Inflammatory Agents, Non-Steroidal/therapeutic useAstragalus PlantCartilage, Articular/drug effects*Cartilage, Articular/metabolismDisease Models, AnimalGlucosides/isolation & purificationGlucosides/pharmacologyGlucosides/therapeutic use*Glycosaminoglycans/metabolismIsoflavones/isolation & purificationIsoflavones/pharmacologyIsoflavones/therapeutic use*Menisci, Tibial/surgeryOsteoarthritis/drug therapy*Piroxicam/therapeutic useProstaglandins/metabolism*Proteins/analysisRabbitsRandom AllocationSynovial Fluid/chemistry*Synovial Fluid/metabolism
DOI
10.1016/j.joca.2007.02.015
PMID
17408983
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
Journal Papers > Research Organization > Cell Therapy Center
AJOU Authors
민, 병현최, 병현
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