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Carboxylate-Containing Two-Photon Probe for the Simultaneous Detection of Extra- and Intracellular pH Values in Colon Cancer Tissue

Authors
Si, HY | Cho, MK | Kang, JS | Noh, CK  | Shin, SJ  | Lim, CS | Kim, HM
Citation
Analytical chemistry, 90(13). : 8058-8064, 2018
Journal Title
Analytical chemistry
ISSN
0003-27001520-6882
Abstract
Acidified extracellular pH (pHe) is directly related to various disorders such as tumor invasion and the resistance to drugs. In this study, we developed two-photon-excitable emission ratiometric probes (XBH1-3) for the in situ measurement of pHe. These probes, based on benzimidazole and polar solubilizing groups, exhibited a strong two-photon-induced fluorescence and sensitive blue-to-green emission color changes with p Ka values of 5.1-5.7. XBH1, containing a carboxylic acid, stained the extracellular region in neutral media: it entered the cell under acidic media, thereby allowing a precise measurement of the extra- and intra-cellular pH values in the acidified tissue. XBH2, containing the sulfonate peripheral unit, facilitated the monitoring of the pHe value only. Ratiometric two-photon microscopy imaging revealed that XBH1 can directly monitor the pH values both inside and outside the cells in colon cancer tissue: there is also the morphological aspect. This could be useful for cancer analyses and drug development.
MeSH

DOI
10.1021/acs.analchem.8b01114
PMID
29847925
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
Ajou Authors
노, 충균  |  신, 성재
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