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Antigen-binding characteristics of circulating IgG autoantibodies to cytokeratin 18 protein in patients with nonallergic asthma.

Authors
Yim, H; Kim, JE; Shin, JY; Ye, YM; Park, HS; Nahm, DH
Citation
Journal of Korean medical science, 21(4):652-655, 2006
Journal Title
Journal of Korean medical science
ISSN
1011-89341598-6357
Abstract
Cytokeratin 18 (CK18) protein was identified as an airway epithelial cell autoantigen associated with nonallergic asthma. Cleavage of CK18 protein by caspase-3 is a marker of early apoptosis in epithelial cells. It has been shown that the expression of active caspase-3 was increased in bronchial epithelial cells of asthmatic patients, when compared with healthy controls. To investigate the antigen-binding characteristics of IgG autoantibodies to CK18 protein in nonallergic asthma, the bindings of IgG autoantibodies to the fragments of CK18 protein cleaved by caspase-3 were analyzed by Western blot using serum samples from three patients with nonallergic asthma. Recombinant human CK18 protein was treated by caspase-3 and cleaved into N-terminal fragment (1-397 amino acids) and C-terminal fragment (398-430 amino acids). The binding capacity of IgG autoantibodies to N-terminal fragment of CK18 was maintained in one patient and reduced in other two patients. IgG autoantibodies from all three patients did not bind to C-terminal fragment of CK 18. In conclusion, IgG autoantibodies to CK18 protein from patients with nonallergic asthma seems to preferentially bind to the whole molecule of CK18 protein and their antigen-binding characteristics were heterogeneous among the patients with nonallergic asthma.
MeSH terms
AdultAgedAntibodies, Monoclonal/immunologyAntibodies, Monoclonal/metabolismAntigen-Antibody ReactionsAsthma/blood*Asthma/immunologyAutoantibodies/bloodAutoantibodies/immunology*Autoantibodies/metabolismBlotting, WesternCaspase 3Caspases/metabolismEpitopes/immunologyEpitopes/metabolismFemaleHumansHydrolysisImmunoglobulin G/bloodImmunoglobulin G/immunology*Immunoglobulin G/metabolismKeratins/chemistryKeratins/immunology*Keratins/metabolismMalePeptide Fragments/immunologyPeptide Fragments/metabolismProtein Binding
DOI
10.3346/jkms.2006.21.4.652
PMID
16891808
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Journal Papers > School of Medicine / Graduate School of Medicine > Pathology
AJOU Authors
임, 현이예, 영민박, 해심남, 동호
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