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Effect of ischemic neuronal insults on amyloid precursor protein processing.

Authors
Lee, PH; Hwang, EM; Hong, HS; Boo, JH; Mook-Jung, I; Huh, K
Citation
Neurochemical research, 31(6):821-827, 2006
Journal Title
Neurochemical research
ISSN
0364-31901573-6903
Abstract
The nature of the association between ischemic stroke and Alzheimer's disease (AD) at the cellular and molecular level is still unknown. We evaluated the effect of ischemic neuronal insults on the regulation of amyloid precursor protein (APP) processing. We used an in vitro model of cerebral ischemia (oxygen-glucose deprivation) to evaluate the effect of ischemic neuronal insults on the amyloidogenic and non-amyloidogenic pathways using human neuroblastoma cell line and primary cultured cells of transgenic mice which expressed human APP (Tg2576). Ischemic neuronal insults increased the production of Abeta in Tg2576 primary culture cells compared to controls. A disintegrin and metalloprotease 10 (ADAM 10) was markedly increased in early stage of ischemic insults, which was followed by decreased level of ADAM 10 expression in later stage. The protein and mRNA expression of beta-site cleavage enzyme (BACE) and BACE activity was not significantly different between the group of ischemic insults and control. By contrast, the activity of gamma-secretase was significantly increased after 4 h of ischemic insults, as compared to controls. The present study showed that the ischemic neuronal insults increased the production of Abeta by influencing APP metabolism, which may link the role of ischemic insults to the pathogenesis of AD.
MeSH terms
Amino Acid SequenceAmyloid/biosynthesisAmyloid Precursor Protein SecretasesAmyloid beta-Protein Precursor/metabolism*AnimalsAspartic Acid EndopeptidasesBase SequenceBrain Ischemia/metabolism*Cell Line, TumorDNA PrimersEndopeptidases/metabolismHumansMiceMice, TransgenicMolecular Sequence DataProtein Processing, Post-Translational*
DOI
10.1007/s11064-006-9086-y
PMID
16794858
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
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