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Peroxisome proliferator-activated receptors-gamma activator, ciglitazone, inhibits human melanocyte growth through induction of apoptosis.

Authors
Kang, HY; Lee, JY; Lee, JS; Choi, YM
Citation
Archives of dermatological research, 297(10):472-476, 2006
Journal Title
Archives of dermatological research
ISSN
0340-36961432-069X
Abstract
Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor superfamily. All three PPAR subtypes, PPAR-alpha, PPAR-beta/delta and PPAR-gamma are expressed in human melanocytes. In this study, we investigated the effects of PPAR-gamma activator on melanocyte growth, and apoptosis. The PPAR-gamma activators ciglitazone, troglitazone, and 15-deoxy-prostaglandin J2 inhibited melanocyte growth in a dose-dependent manner. This inhibitory effect of ciglitazone seemed to occur through induction of apoptosis. Apoptosis was increased after ciglitazone treatment, which was observed by the TUNEL method and flow cytometry. We noted a decrease in extracellular signal regulated kinase protein expression under ciglitazone treatment. Western blot analysis revealed an apparent time-dependent reduction in Bcl-2 protein levels in ciglitazone-treated melanocytes. In terms of Bax expression, a difference was not found. The expression of caspase-3 proteins was increased time-dependently with ciglitazone treatment. These results indicate that melanocyte growth and apoptosis may be modulated through PPAR-gamma and that ciglitazone, a PPAR-gamma activator, inhibits growth of human melanocytes by inducing apoptosis.
MeSH terms
Apoptosis/drug effects*Apoptosis/physiologyCaspase 3Caspases/geneticsCaspases/metabolismCell Proliferation/drug effects*Cells, CulturedChromans/pharmacologyDose-Response Relationship, DrugGene Expression Regulation/drug effectsHumansMaleMelanocytes/cytologyMelanocytes/drug effectsMelanocytes/physiology*PPAR gamma/physiology*Prostaglandin D2/analogs & derivativesProstaglandin D2/pharmacologyProto-Oncogene Proteins c-bcl-2/geneticsProto-Oncogene Proteins c-bcl-2/metabolismThiazolidinediones/pharmacology*bcl-2-Associated X Protein/geneticsbcl-2-Associated X Protein/metabolism
DOI
10.1007/s00403-006-0646-4
PMID
16474974
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Dermatology
AJOU Authors
강, 희영
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