161 335

Cited 13 times in

Discordant genetic changes in ovarian and endometrial endometrioid carcinomas: a potential pitfall in molecular diagnosis.

Authors
Chang, KH; Albarracin, C; Luthra, R; Wang, L; Zheng, W; Malpica, A; Deavers, MT; Silva, EG; Liu, J
Citation
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, 16(1):178-182, 2006
Journal Title
International journal of gynecological cancer : official journal of the International Gynecological Cancer Society
ISSN
1048-891X1525-1438
Abstract
Endometrioid carcinoma simultaneously involving ovaries as well as the uterine corpus may present a diagnostic dilemma because of the difficulty in determining whether the lesions are separate primary tumors or metastases. It has been reported that the detection of clonality using microsatellite markers may be useful in solving this dilemma. To determine the usefulness of this technique, we compared the genetic alterations in microsatellite markers present in matched pairs of ovarian tumors from 12 patients. The study includes four ovarian cancer FIGO stage I and eight stage III/IV patients, and four patients also with independent endometrial carcinoma of the uterus. DNA from paraffin-embedded tissue was extracted and amplified using a multiplex polymerase chain reaction, after which the status of microsatellite instability and loss of heterozygosity in four microsatellite loci (BAT25, BAT26, D17S250, and D5S346) were determined. In the four patients with stage I ovarian cancer, four microsatellite markers were identical in one patient and three were identical in the remaining three patients. In high-stage patients, three markers were identical in at least 4/8 cases. In three of four patients with uterine involvement, three of the four markers were identical in the uterine tumor and one of the corresponding ovarian tumors. These results suggest that genetic discordance does not indicate independent origin or metastasis of the tumor but instead a progression of genetic changes at separate sites probably due to the marked genetic instability existing in these tumors. Because of these discordant genetic changes, great caution should be taken when distinguishing between primary and metastatic tumors on the basis of microsatellite markers.
MeSH terms
AdultAgedBiopsy, NeedleDNA Sequence, Unstable*DNA, Neoplasm/analysisEndometrial Neoplasms/genetics*Endometrial Neoplasms/pathologyFemaleGene Expression Regulation, NeoplasticHumansImmunohistochemistryMicrosatellite Repeats*Middle AgedMolecular BiologyNeoplasm StagingNeoplasms, Multiple Primary/genetics*Neoplasms, Multiple Primary/pathologyOvarian Neoplasms/genetics*Ovarian Neoplasms/pathologySampling StudiesSensitivity and SpecificityTissue Culture Techniquesbeta Catenin/genetics
DOI
10.1111/j.1525-1438.2006.00293.x
PMID
16445630
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Medical Science
AJOU Authors
장, 기홍
Files in This Item:
Full-Text Not Available.txtDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse