Cited 0 times in Scipus Cited Count

The growth of brain tumors can be suppressed by multiple transplantation of mesenchymal stem cells expressing cytosine deaminase.

DC Field Value Language
dc.contributor.authorChang, DY-
dc.contributor.authorYoo, SW-
dc.contributor.authorHong, Y-
dc.contributor.authorKim, S-
dc.contributor.authorKim, SJ-
dc.contributor.authorYoon, SH-
dc.contributor.authorCho, KG-
dc.contributor.authorPaek, SH-
dc.contributor.authorLee, YD-
dc.contributor.authorKim, SS-
dc.contributor.authorSuh-Kim, H-
dc.date.accessioned2011-04-26T04:25:02Z-
dc.date.available2011-04-26T04:25:02Z-
dc.date.issued2010-
dc.identifier.issn0020-7136-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/2432-
dc.description.abstractSuicide genes have recently emerged as an attractive alternative therapy for the treatment of various types of intractable cancers. The efficacy of suicide gene therapy relies on efficient gene delivery to target tissues and the localized concentration of final gene products. Here, we showed a potential ex vivo therapy that used mesenchymal stem cells (MSCs) as cellular vehicles to deliver a bacterial suicide gene, cytosine deaminase (CD) to brain tumors. MSCs were engineered to produce CD enzymes at various levels using different promoters. When co-cultured, CD-expressing MSCs had a bystander, anti-cancer effect on neighboring C6 glioma cells in proportion to the levels of CD enzymes that could convert a nontoxic prodrug, 5-fluorocytosine (5-FC) into cytotoxic 5-fluorouracil (5-FU) in vitro. Consistent with the in vitro results, for early stage brain tumors induced by intracranial inoculation of C6 cells, transplantation of CD-expressing MSCs reduced tumor mass in proportion to 5-FC dosages. However, for later stage, established tumors, a single treatment was insufficient, but only multiple transplantations were able to successfully repress tumor growth. Our findings indicate that the level of total CD enzyme activity is a critical parameter that is likely to affect the clinical efficacy for CD gene therapy. Our results also highlight the potential advantages of autograftable MSCs compared with other types of allogeneic stem cells for the treatment of recurrent glioblastomas through repetitive treatments.-
dc.language.isoen-
dc.subject.MESHAdolescent-
dc.subject.MESHAnimals-
dc.subject.MESHBrain Neoplasms-
dc.subject.MESHBystander Effect-
dc.subject.MESHChild-
dc.subject.MESHChromatography, High Pressure Liquid-
dc.subject.MESHCytosine Deaminase-
dc.subject.MESHFlucytosine-
dc.subject.MESHFluorouracil-
dc.subject.MESHGene Therapy-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHMesenchymal Stem Cell Transplantation-
dc.subject.MESHMesenchymal Stem Cells-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHTumor Cells, Cultured-
dc.titleThe growth of brain tumors can be suppressed by multiple transplantation of mesenchymal stem cells expressing cytosine deaminase.-
dc.typeArticle-
dc.identifier.pmid20473873-
dc.contributor.affiliatedAuthor장, 다영-
dc.contributor.affiliatedAuthor유, 승완-
dc.contributor.affiliatedAuthor김, 세중-
dc.contributor.affiliatedAuthor조, 경기-
dc.contributor.affiliatedAuthor이, 영돈-
dc.contributor.affiliatedAuthor김, 성수-
dc.contributor.affiliatedAuthor서, 해영-
dc.type.localJournal Papers-
dc.identifier.doi10.1002/ijc.25383-
dc.citation.titleInternational journal of cancer-
dc.citation.volume127-
dc.citation.number8-
dc.citation.date2010-
dc.citation.startPage1975-
dc.citation.endPage1983-
dc.identifier.bibliographicCitationInternational journal of cancer, 127(8). : 1975-1983, 2010-
dc.identifier.eissn1097-0215-
dc.relation.journalidJ000207136-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
Journal Papers > School of Medicine / Graduate School of Medicine > Urology
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
Files in This Item:
There are no files associated with this item.

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse