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An RNA-hydrolyzing recombinant antibody exhibits an antiviral activity against classical swine fever virus.

Authors
Jun, HR; Pham, CD; Lim, SI; Lee, SC; Kim, YS; Park, S; Kwon, MH
Citation
Biochemical and biophysical research communications, 395(4):484-489, 2010
Journal Title
Biochemical and biophysical research communications
ISSN
0006-291X1090-2104
Abstract
Some proteins with ribonuclease (RNase) activity have been shown to suppress viral replication. A well-characterized recombinant antibody, 3D8 single-chain variable fragment (3D8 scFv), has RNA-hydrolyzing and cell-penetrating activities. Here, we investigated antiviral activity of 3D8 scFv against classical swine fever virus (CSFV). In a cell line expressing 3D8 scFv (C26), intracellular RNA-hydrolysis activity was higher compared to control PK-15 cells and viral replication was strongly suppressed at the viral RNA level, with the evidence of independency of IFN-alpha/beta induction. Exogenous treatment of 3D8 scFv, prior to or post-CSFV infection, was also shown to suppress CSFV replication at the viral RNA level. These observations suggest that antiviral activity of 3D8 scFv may be due to the intrinsic RNase activity of 3D8 scFv, which is capable of targeting viral RNA genomes or transcripts.
MeSH terms
AnimalsAntibodies, Antinuclear/metabolismAntibodies, Antinuclear/pharmacology*Antibodies, Monoclonal/metabolismAntibodies, Monoclonal/pharmacology*Antiviral Agents/metabolismAntiviral Agents/pharmacology*Cell LineClassical Swine Fever/immunologyClassical Swine Fever/virology*Classical swine fever virus/drug effects*Down-RegulationHydrolysisInterferon Type I/immunologyRNA, Viral/immunologyRNA, Viral/metabolismRecombinant Proteins/metabolismRecombinant Proteins/pharmacology*Ribonucleases/metabolismRibonucleases/pharmacologySingle-Chain Antibodies/immunologySingle-Chain Antibodies/pharmacology*SwineVirus Replication/drug effects
DOI
10.1016/j.bbrc.2010.04.032
PMID
20382124
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
박선권명희
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