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The loss of phenol sulfotransferase 1 in hepatocellular carcinogenesis.

Authors
Yeo, M; Na, YM; Kim, DK; Kim, YB; Wang, HJ; Lee, JA; Cheong, JY; Lee, KJ; Paik, YK; Cho, SW
Citation
Proteomics, 10(2):266-276, 2010
Journal Title
Proteomics
ISSN
1615-98531615-9861
Abstract
Biomarkers for the detection of early hepatocellular carcinoma (HCC) are urgently needed. To identify biomarkers of HCC, we performed a comparative proteomics analysis, based on 2-DE of HCC tissues and surrounding non-tumor tissues. Six xenobiotic enzymes were significantly down-regulated in the HCC tissue. Among these, phenol sulfotransferase (SULT1A1) was confirmed by Western blot analysis in 105 HCC patients. SULT1A1 showed a significant decrease in 98.1% of the HCC tissues, with 88.6% sensitivity and 66.7% specificity for the detection of HCC. Immunohistochemistry for SULT1A1 was performed and compared with glypican-3, which is a well-known marker of HCC. The results showed down-regulation of SULT1A1 and up-regulation of glypican-3 in 52.6 and 71.9% of the HCCs, and the use of both markers improved the sensitivity up to 78.9%. Moreover, SULT1A1 was useful in differentiating early HCC from benign dysplastic nodules. Clinically, the down-regulation of SULT1A1 was closely associated with an advanced International Union Against Cancer stage and high levels of serum alpha-fetoprotein. In conclusion, the results of this study demonstrate that the loss of SULT1A1 appears to be a characteristic molecular signature of HCC. SULT1A1 might be a useful biomarker for the detection of early HCC and help predict the clinical outcome of patients with HCC.
MeSH terms
Amino Acid SequenceArylsulfotransferase/chemistry/*metabolismCarcinoma, Hepatocellular/blood/*enzymology/pathology*Down-RegulationElectrophoresis, Gel, Two-DimensionalFemaleGlypicans/metabolismHumansLiver Neoplasms/*enzymology/pathologyMaleMiddle AgedNeoplasm StagingTumor Markers, Biological/chemistry/*metabolismalpha-Fetoproteins/metabolism
DOI
10.1002/pmic.200900721
PMID
19904771
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
Journal Papers > Research Organization > Genomic Research Center for Gastroenterology
Journal Papers > School of Medicine / Graduate School of Medicine > Pathology
Journal Papers > School of Medicine / Graduate School of Medicine > Surgery
AJOU Authors
여말희김영배왕희정정재연이광재조성원
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