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Imiquimod induces apoptosis of human melanocytes.

Authors
Kim, CH; Ahn, JH; Kang, SU; Hwang, HS; Lee, MH; Pyun, JH; Kang, HY
Citation
Archives of dermatological research, 302(4):301-306, 2010
Journal Title
Archives of dermatological research
ISSN
0340-36961432-069X
Abstract
Development of vitiligo-like hypopigmentary lesions associated with topical imiquimod has been reported. We hypothesized that mode of action of imiquimod in melanocytes may include triggering of apoptosis resulted in loss of cells, which may be a possible mechanism of imiquimod-induced hypopigmentary lesions. Therefore, we investigated whether imiquimod induces apoptosis of human melanocytes and also whether it modulates expression of apoptosis-related molecules in human melanocytes. Imiquimod treatment induced apoptosis of melanocytes, which was observed by TUNEL assay and Hoechst 33258 staining. Imiquimod-induced apoptosis was further shown by measuring mitochondrial membrane potential in melanocytes. The apoptotic activity of imiquimod was associated with caspase-3, Bcl-2 and mitogen-activated protein kinase expression in melanocytes. These results indicated that imiquimod induces apoptosis of melanocytes. These findings may provide a clue to understand pathogenesis of imiquimod-induced vitiligo-like hypopigmentary lesions.
MeSH terms
Adjuvants, Immunologic/adverse effectsAminoquinolines/adverse effects*Apoptosis/drug effectsCaspase 3/metabolismCells, CulturedHumansHypopigmentationIn Situ Nick-End LabelingMelanocytes/drug effects*Melanocytes/metabolismMelanocytes/pathologyMembrane Potential, Mitochondrial/drug effectsMitogen-Activated Protein Kinases/metabolismProto-Oncogene Proteins c-bcl-2/metabolismVitiligo/chemically induced*Vitiligo/metabolismVitiligo/pathologyVitiligo/physiopathology
DOI
10.1007/s00403-009-1012-0
PMID
20033192
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Dermatology
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
AJOU Authors
김, 철호강, 희영
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