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Prevention of intraperitoneal adhesions and abscesses by polysaccharides isolated from Phellinus spp in a rat peritonitis model.

Bae, JS; Ahn, SJ; Yim, H; Jang, KH; Jin, HK
Annals of surgery, 241(3):534-540, 2005
Journal Title
Annals of surgery
OBJECTIVE: To assess whether polysaccharides isolated from fungi, Phellinus spp, could reduce the adhesion and abscess formation in a rat peritonitis model.

SUMMARY BACKGROUND DATA: Although polysaccharides from Phellinus spp is a well-known material with antiinflammatory properties, little is known regarding its ability to prevent intraperitoneal adhesions. We have assessed the adhesion- and abscess-reducing effect of polysaccharides from Phellinus gilvus (PG) and Phellinus linteus (PL) in a rat peritonitis model.

METHODS: In 60 SD rats, experimental peritonitis was induced using the cecal ligation and puncture model (CLP). Animals were randomly assigned to 5 groups; ringer lactate solution (RL group), polysaccharides from PG and PL (PG and PL group), hyaluronic acid (HA group), and carboxymethylcellulose (CMC group). Intraperitoneal adhesions and abscesses were noted at 7 day after CLP. RT-PCR assay for urokinase-type plasminogen activator (uPA), its cellular receptor (uPAR), tissue-type plasminogen activator (tPA), plasminogen activator inhibitor type 1 (PAI-1), and tumor necrosis factor (TNF)- alpha was performed to assess the cecal tissue.

RESULTS: Adhesion formation was significantly reduced in PG, PL, CMC, and HA groups (P < 0.001) compared with that in RL group. The incidence of abscesses was also significantly reduced in PG and PL groups (P < 0.05) compared with that in the RL group. The level of uPA, uPAR, tPA, and TNF-alpha was highly expressed in PG and PL group, as compared with the RL group.

CONCLUSIONS: We concluded that PG and PL had significant adhesion- and abscess-reducing effects and may act by modulating fibrinolytic capacity of uPA and/or tPA produced from macrophages in a rat peritonitis model.
MeSH terms
Abdominal Abscess/etiologyAbdominal Abscess/microbiologyAbdominal Abscess/prevention & control*AnimalsAscitic Fluid/microbiologyBasidiomycota*Carboxymethylcellulose Sodium/therapeutic useCecum/chemistryHyaluronic Acid/therapeutic useMalePeritonitis/complications*Peritonitis/metabolismPeritonitis/microbiologyPlasminogen Activator Inhibitor 1/analysisPolysaccharides/therapeutic use*RatsRats, Sprague-DawleyReceptors, Cell Surface/analysisReceptors, Urokinase Plasminogen ActivatorReverse Transcriptase Polymerase Chain ReactionTissue Adhesions/etiologyTissue Adhesions/pathologyTissue Adhesions/prevention & control*Tissue Plasminogen Activator/analysisTumor Necrosis Factor-alpha/analysisUrokinase-Type Plasminogen Activator/analysis
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Journal Papers > School of Medicine / Graduate School of Medicine > Pathology
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임, 현이
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