32 243

Cited 52 times in

Polymorphism of tandem repeat in promoter of 5-lipoxygenase in ASA-intolerant asthma: a positive association with airway hyperresponsiveness.

Authors
Kim, SH; Bae, JS; Suh, CH; Nahm, DH; Holloway, JW; Park, HS
Citation
Allergy, 60(6):760-765, 2005
Journal Title
Allergy
ISSN
0105-45381398-9995
Abstract
BACKGROUND: 5-Lipooxygenase (ALOX5) and 5-lipoxygenase-activating protein (ALOX5AP) are known as key enzymes in cysteinyl-leukotriene (cys-LT) production, critical mediators in aspirin acetylsalicyclic acid (ASA)-intolerant asthma (AIA). To date, studies of the promoter region of ALOX5 gene has revealed the potential influence of a variable number of tandem repeats of a Sp1- and Egr1-binding motif, on the transcription rate.



METHODS: To understand the pathological process that arises from cys-LT overproduction in AIA, we genotyped ALOX5 Sp1 and ALOX5AP poly(A) repeat promoter polymorphism by fluorescent-based capillary electrophoresis in the Korean population.



RESULTS: No significant differences in allele and genotype frequencies of the ALOX5 and ALOX5AP promoter polymorphisms were observed between the three groups. However, there was a strong association of the ALOX5 Sp1 repeat polymorphism with airway hyperresponsiveness (AHR; PC20 methacholine); AIA patients carrying a mutant allele (n > 5 or n < 5 repeats) showed increased AHR compared to AIA patients with wild-type genotype (P=0.003).



CONCLUSION: Although the alleles of the ALOX5 and ALOX5AP promoter cannot be considered as a prominent risk factor in the development of AIA, the genetic variant of tandem repeat (GGGCGG; Sp1-binding motif) in ALOX5 promoter is associated with the severity of airway hyperresponsiveness in AIA patients.
MeSH terms
5-Lipoxygenase-Activating ProteinsArachidonate 5-Lipoxygenase/genetics*Aspirin/adverse effects*Asthma/epidemiologyAsthma/genetics*Carrier Proteins/geneticsDrug Hypersensitivity/epidemiologyDrug Hypersensitivity/genetics*FemaleHumansKorea/epidemiologyMaleMembrane Proteins/geneticsPolymorphism, Genetic*Promoter Regions, GeneticTandem Repeat Sequences
DOI
10.1111/j.1398-9995.2005.00780.x
PMID
15876305
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Journal Papers > Research Organization > Regional Clinical Trial Center
Journal Papers > School of Medicine / Graduate School of Medicine > Rheumatology
AJOU Authors
김, 승현서, 창희남, 동호박, 해심
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse