56 150

Cited 0 times in

Role of neuropeptide Y and proopiomelanocortin in fluoxetine-induced anorexia.

Authors
Myung, CS; Kim, BT; Choi, SH; Song, GY; Lee, SY; Jahng, JW
Citation
Archives of pharmacal research, 28(6):716-721, 2005
Journal Title
Archives of pharmacal research
ISSN
0253-6269
Abstract
Fluoxetine is an anorexic agent known to reduce food intake and weight gain. However, the molecular mechanism by which fluoxetine induces anorexia has not been well-established. We examined mRNA expression levels of neuropeptide Y (NPY) and proopiomelanocortin (POMC) in the brain regions of rats using RT-PCR and in situ hybridization techniques after 2 weeks of administering fluoxetine daily. Fluoxetine persistently suppressed food intake and weight gain during the experimental period. The pair-fed group confirmed that the reduction in body weight in the fluoxetine treated rats resulted primarily from decreased food intake. RT-PCR analyses showed that mRNA expression levels of both NPY and POMC were markedly reduced by fluoxetine treatment in all parts of the brain examined, including the hypothalamus. POMC mRNA in situ signals were significantly decreased, NPY levels tended to increase in the arcuate nucleus (ARC) of fluoxetine treated rats (compared to the vehicle controls). In the pair-fed group, NPY mRNA levels did not change, but the POMC levels decreased (compared with the vehicle controls). These results reveal that the chronic administration of fluoxetine decreases expression levels in both NPY and POMC in the brain, and suggests that fluoxetine-induced anorexia may not be mediated by changes in the ARC expression of either NPY or POMC. It is possible that a fluoxetine raised level of 5-HT play an inhibitory role in the orectic action caused by a reduced expression of ARC POMC (alpha-MSH).
MeSH terms
AnimalsAnorexia/chemically inducedAnorexia/metabolism*Appetite Regulation/drug effects*Arcuate Nucleus/chemistryArcuate Nucleus/metabolism*AutoradiographyBody Weight/drug effectsBrain Chemistry/drug effectsEating/drug effectsFluoxetine*Gene Expression Regulation/drug effectsMaleNeuropeptide Y/biosynthesisNeuropeptide Y/metabolism*Pro-Opiomelanocortin/biosynthesisPro-Opiomelanocortin/metabolism*RNA/isolation & purificationRNA, Messenger/metabolismRatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionSerotonin Uptake Inhibitors*
PMID
16042082
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Family Practice & Community Health
AJOU Authors
김, 범택
Files in This Item:
Full-Text Not Available.txtDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse