Cited 0 times in Scipus Cited Count

Tumor-specific gene therapy for uterine cervical cancer using MN/CA9-directed replication-competent adenovirus.

DC Field Value Language
dc.contributor.authorLim, HY-
dc.contributor.authorAhn, M-
dc.contributor.authorChung, HC-
dc.contributor.authorGardner, TA-
dc.contributor.authorKao, C-
dc.contributor.authorLee, SJ-
dc.contributor.authorKim, SJ-
dc.date.accessioned2011-07-12T05:59:50Z-
dc.date.available2011-07-12T05:59:50Z-
dc.date.issued2004-
dc.identifier.issn0929-1903-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3286-
dc.description.abstractAlthough gene therapies using tissue-specific promoters have been reported to be a promising tool for treating cancers, few studies have explored this possibility for uterine cervical cancer. MN/CA9 is a transmembrane glycoprotein that was first identified in the human cervical carcinoma cell line, HeLa. Since MN/CA9 protein is highly expressed in uterine cervical cancer tissues, but not in normal cervix, we constructed a tumor-specific replication-competent adenoviral vector utilizing MN/CA9 promoter (Ad-MN/CA9-E1a), which can replicate only in MN/CA9-expressing cells. Infection of Ad-MN/CA9-E1a to MN/CA9-positive uterine cervical cancer cells (HeLa, C-33 A and SiHa) resulted in much stronger Ad5 E1a protein expressions compared with MN/CA9-negative cells (SK-RC-29), suggesting a tissue-specific replication of this recombinant adenovirus. In vitro cytotoxicity assay revealed that the growth of MN/CA9-positive cells was significantly inhibited with 0.01-1 MOI of Ad-MN/CA9-E1a, but the growth of MN/CA9-negative cells (SK-RC-29) could only be inhibited by as many as 100 MOI. Intratumoral injection of Ad-MN/CA9-E1a effectively induced growth delay of HeLa tumors in nude mice. These results suggest that a novel replication-competent adenoviral vector mediated by MN/CA9 promoter, Ad-MN/CA9-E1a, can selectively replicate in MN/CA9-expressing tumors with cytotoxic effects and may be utilized for the treatment of uterine cervical cancer.-
dc.language.isoen-
dc.subject.MESHAdenoviridae-
dc.subject.MESHAdenovirus E1A Proteins-
dc.subject.MESHAnimals-
dc.subject.MESHAntigens, Neoplasm-
dc.subject.MESHCarbonic Anhydrases-
dc.subject.MESHFemale-
dc.subject.MESHGene Expression-
dc.subject.MESHGene Therapy-
dc.subject.MESHGenetic Vectors-
dc.subject.MESHHela Cells-
dc.subject.MESHHumans-
dc.subject.MESHMice-
dc.subject.MESHPromoter Regions, Genetic-
dc.subject.MESHRNA, Messenger-
dc.subject.MESHUterine Cervical Neoplasms-
dc.subject.MESHVirus Replication-
dc.subject.MESHXenograft Model Antitumor Assays-
dc.titleTumor-specific gene therapy for uterine cervical cancer using MN/CA9-directed replication-competent adenovirus.-
dc.typeArticle-
dc.identifier.pmid15167900-
dc.contributor.affiliatedAuthor임, 호영-
dc.contributor.affiliatedAuthor김, 세중-
dc.type.localJournal Papers-
dc.identifier.doi10.1038/sj.cgt.7700732-
dc.citation.titleCancer gene therapy-
dc.citation.volume11-
dc.citation.number8-
dc.citation.date2004-
dc.citation.startPage532-
dc.citation.endPage538-
dc.identifier.bibliographicCitationCancer gene therapy, 11(8). : 532-538, 2004-
dc.identifier.eissn1476-5500-
dc.relation.journalidJ009291903-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Hematology-Oncology
Journal Papers > School of Medicine / Graduate School of Medicine > Urology
Files in This Item:
There are no files associated with this item.

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse