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Endophilin B1 is required for the maintenance of mitochondrial morphology.

Authors
Karbowski, M; Jeong, SY; Youle, RJ
Citation
The Journal of cell biology, 166(7):1027-1039, 2004
Journal Title
The Journal of cell biology
ISSN
0021-95251540-8140
Abstract
We report that a fatty acyl transferase, endophilin B1, is required for maintenance of mitochondrial morphology. Down-regulation of this protein or overexpression of endophilin B1 lacking the NH(2)-terminal lipid-modifying domain causes striking alterations of the mitochondrial distribution and morphology. Dissociation of the outer mitochondrial membrane compartment from that of the matrix, and formation of vesicles and tubules of outer mitochondrial membrane, was also observed in both endophilin B1 knockdown cells and after overexpression of the truncated protein, indicating that endophilin B1 is required for the regulation of the outer mitochondrial membrane dynamics. We also show that endophilin B1 translocates to the mitochondria during the synchronous remodeling of the mitochondrial network that has been described to occur during apoptosis. Double knockdown of endophilin B1 and Drp1 leads to a mitochondrial phenotype identical to that of the Drp1 single knockdown, a result consistent with Drp1 acting upstream of endophilin B1 in the maintenance of morphological dynamics of mitochondria.
MeSH terms
Acyltransferases/genetics/metabolism/*physiologyAnimalsApoptosis/geneticsCOS CellsDown-Regulation/geneticsEnergy Metabolism/*geneticsGTP Phosphohydrolases/geneticsHela CellsHumansIntracellular Membranes/*metabolism/ultrastructureMicrotubule-Associated Proteins/geneticsMitochondria/genetics/*metabolism/ultrastructureMitochondrial ProteinsPhenotypeProtein Transport/geneticsRNA Interference
DOI
10.1083/jcb.200407046
PMID
15452144
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Medical Genetics
AJOU Authors
정, 선용
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